Salivary Gland Transcriptomes and Proteomes of Phlebotomus tobbi and Phlebotomus sergenti, Vectors of Leishmaniasis 英文参考文献.docVIP

下载本文档

8
0
约9.71万字
约 27页
2017-05-13 发布于上海
举报
版权申诉

Salivary Gland Transcriptomes and Proteomes of Phlebotomus tobbi and Phlebotomus sergenti, Vectors of Leishmaniasis 英文参考文献.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Salivary Gland Transcriptomes and Proteomes of Phlebotomus tobbi and Phlebotomus sergenti, Vectors of Leishmaniasis 英文参考文献

SalivaryGlandTranscriptomesandProteomesof PhlebotomustobbiandPhlebotomussergenti,Vectors ofLeishmaniasis IvaRohousˇova′1,SreenathSubrahmanyam2,VeˇraVolfova′1,JianbingMu3,PetrVolf1, JesusG.Valenzuela2*,RyanC.Jochim2* 1Department ofParasitology,FacultyofScience,CharlesUniversityinPrague,Prague,CzechRepublic, 2VectorMolecularBiology Section,LaboratoryofMalariaand Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland, United States of America, 3Malaria Genomics Section,LaboratoryofMalariaandVectorResearch,NationalInstituteofAllergyandInfectiousDiseases,NationalInstitutesofHealth,Rockville,Maryland,UnitedStatesof America Abstract Background: Phlebotomus tobbi is a vector of Leishmania infantum, and P. sergenti is a vector of Leishmania tropica. Le. infantumandLe.tropicatypicallycausevisceralorcutaneousleishmaniasis,respectively,butLe.infantumstrainstransmitted byP.tobbicancausecutaneousdisease.Tobetterunderstandthecomponentsandpossibleimplicationsofsandflysaliva inleishmaniasis,thetranscriptomesofthesalivaryglands(SGs)ofthesetwosandflyspeciesweresequenced,characterized andcompared. Methodology/PrincipalFindings:cDNAlibrariesofP.tobbiandP.sergentifemaleSGswereconstructed,sequenced,and analyzed.Clones(1,152)wererandomlypickedfromeachlibrary,producing1,142high-qualitysequencesfromP.tobbiand 1,090 from P. sergenti. The most abundant, secreted putative proteins were categorized as antigen 5-related proteins, apyrases, hyaluronidases, D7-related and PpSP15-like proteins, ParSP25-like proteins, PpSP32-like proteins, yellow-related proteins, the 33-kDa salivary proteins, and the 41.9-kDa superfamily of proteins. Phylogenetic analyses and multiple sequence alignments of putative proteins were used to elucidate molecular evolution and describe conserved domains, activesites,andcatalyticresidues.ProteomicanalysesofP.tobbiandP.sergentiSGswereusedtoconfirmtheidentification of35full-lengthsequences(18inP.to