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Salivary testosterone levels in preadolescent children 英文参考文献.doc

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BMC Pediatrics BioMedCentral BMC2002Pediatrics, 2 Research article x Salivary testosterone levels in preadolescent children Daniela Ostatníková*1, Karol Pastor2, Zdenek Putz3, Monika Dohnányiová1, Anna Mata?eje1 and Richard Hampl4 Address: 1Institute of Physiology, School of Medicine, Comenius University, Bratislava, Slovak Republic, 2Department of Probability and Statistics, Faculty of Mathematics and Physics, Comenius University, Bratislava, Slovak Republic, 3Institute of Endocrinology, Lubochòa, Slovak Republic and 4Institute of Endocrinology, Praha, Czech Republic E-mail: Daniela Ostatníková* - ostatnikova@fmed.uniba.sk; Karol Pastor - karol.pastor@fmph.uniba.sk; Zdenek Putz - putz@viapvt.sk; Monika Dohnányiová - dohnanyiovam@slofa.sk; Anna Mata?eje - mataseje@fmad.uniba.sk; Richard Hampl - rhampl@endo.cz *Corresponding author Published: 3 June 2002 Received: 4 March 2002 Accepted: 3 June 2002 BMC Pediatrics 2002, 2:5 This article is available from: /1471-2431/2/5 ? 2002 Ostatníková et al; licensee BioMed Central Ltd. Verbatim copying and redistribution of this article are permitted in any medium for any purpose, provided this notice is preserved along with the articles original URL. Abstract Background: Saliva reflects the plasma free fraction of testosterone which is biologically active, and available for uptake by tissues. Testosterone concentration in saliva, though differing slightly from the concentration of unbound testosterone in serum, is in good correlation with the latter, indicating that salivary testosterone provides a reliable method for determination of serum free testosterone. The study aimed to investigate salivary testosterone levels and their changes in preadolescent children and to study sexual dimorphism. Methods: Testosterone levels were determined in 203 healthy preadolescent children (77 girls and 126 boys) from saliva samples by radioimmunoassay. Sampling was performed once a year with respec