SCS3 and YFT2 Link Transcription of Phospholipid Biosynthetic Genes to ER Stress and the UPR 英文参考文献.docVIP

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SCS3 and YFT2 Link Transcription of Phospholipid Biosynthetic Genes to ER Stress and the UPR 英文参考文献.doc

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SCS3 and YFT2 Link Transcription of Phospholipid Biosynthetic Genes to ER Stress and the UPR 英文参考文献

SCS3andYFT2LinkTranscriptionofPhospholipid BiosyntheticGenestoERStressandtheUPR RobynD.Moir1,DavidA.Gross1,2,DavidL.Silver1,2*,IanM.Willis1,3 * 1Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York, United States of America, 2Signature Research Program in Cardiovascular and MetabolicDisorders,Duke–NUSGraduateMedicalSchoolSingapore,Singapore,Singapore,3DepartmentofSystemsandComputationalBiology,AlbertEinsteinCollege ofMedicine,Bronx,NewYork,UnitedStatesofAmerica Abstract Theability tostorenutrients inlipiddroplets(LDs) is anancientfunctionthatprovides theprimarysourceofmetabolic energyduringperiodsofnutrientinsufficiencyandbetweenmeals.TheFatstorage-InducingTransmembrane(FIT)proteins are conserved ER–resident proteins that facilitate fat storage by partitioning energy-rich triglycerides into LDs. FIT2, the ancientorthologoftheFITgenefamilyfirstidentifiedinmammalshastwohomologsinSaccharomycescerevisiae(SCS3and YFT2)andotherfungioftheSaccharomycotinalineage.Despitethecoevolutionofthesegenesformorethan170million yearsandtheirdivergencefromhighereukaryotes,SCS3,YFT2,andthehumanFIT2generetainsomecommonfunctions: expressionoftheyeastgenesinahumanembryonickidneycelllinepromotesLDformation,andexpressionofhumanFIT2 inyeastrescuestheinositolauxotrophyandchemicalandgeneticphenotypesofstrainslackingSCS3.Tobetterunderstand thefunctionofSCS3andYFT2,weinvestigatedthechemicalsensitivitiesofstrainsdeletedforeitherorbothgenesand identifiedsyntheticgeneticinteractionsagainsttheviableyeastgene-deletioncollection.WeshowthatSCS3andYFT2have shared and unique functions that connect major biosynthetic processes critical for cell growth. These include lipid metabolism,vesiculartrafficking,transcriptionofphospholipidbiosyntheticgenes,andproteinsynthesis.Thegeneticdata indicate that optimal strain fitness requires a balance between phospholipid synthesis and protein synthesis and that deletion of SCS3 and YFT2 impacts a regulatory mechanism that coordinates the