Screening of Filamentous Fungi to Identify Biocatalysts for Lupeol Biotransformation 英文参考文献.docVIP

Molecules 2010, 15, 6140-6151; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Communication Screening of Filamentous Fungi to Identify Biocatalysts for Lupeol Biotransformation Tatiane C. de Carvalho 1, Aline M. Polizeli 1, Izabel C. C. Turatti 1, Marcela E. Severiano 2, Carlos E. de Carvalho 2, Sérgio R. Ambrósio 2, Ant?nio E. M. Crotti 2, Uir S. de Figueiredo 3, Paulo C. Vieira 4 and Niege A. J. C. Furtado 1,* 1 Faculdade de Ciências Farmacêuticas de Ribeir?o Preto, Universidade de S?o Paulo, Ribeir?o Preto, SP, 14040-903, Brazil; E-Mails: carvalho_tc@ .br (T.C.C.); alinepolizeli@ (A.M.P.); izcristu@usp.br (I.C.C.T.) 2 Núcleo de Pesquisa em Ciências Exatas e Tecnológicas, Universidade de Franca, Franca, SP, 14404-600, Brazil; E-Mails: marcellaes@ (M.E.S.); denossauro7@ (C.E.C.); sergioambrosio@unifran.br (S.R.A.); millercrotti@unifran.br (A.E.M.C.) 3 Instituto de Ciências Exatas e da Terra, Universidade Federal de Mato Grosso, MT, 78060-900, Brazil; E-Mail: uirs@.br (U.S.F.) 4 Centro de Ciências Exatas e de Tecnologia, Universidade Federal de S?o Carlos, SP, 13565-905, Brazil; E-Mail: paulo@dq.ufscar.br (P.C.V.) * Author to whom correspondence should be addressed; E-Mail: niege@fcfrp.usp.br; Tel.: +55-16 Fax: +55-16 Received: 11 August 2010; in revised form: 30 August 2010 / Accepted: 30 August 2010 / Published: 1 September 2010 Abstract: The goal of the study was to evaluate the ability of filamentous fungi to biotransform the pentacyclic triterpene lupeol. The microbial transformations were carried out in shake flasks in different media. Experiments were also run with control flasks. Samples of each culture were taken every 24 hours, extracted with ethyl acetate, and analyzed by GC-MS. The biotransformation of lupeol by Aspergillus ochraceus and Mucor rouxii afforded two compounds in each culture, which were detected in the cultures de