Selecting Temperature for Protein Crystallization Screens Using the Temperature Dependence of the Second Virial Coefficient 英文参考文献.docVIP

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Selecting Temperature for Protein Crystallization Screens Using the Temperature Dependence of the Second Virial Coefficient 英文参考文献.doc

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Selecting Temperature for Protein Crystallization Screens Using the Temperature Dependence of the Second Virial Coefficient 英文参考文献

SelectingTemperatureforProteinCrystallizationScreens UsingtheTemperatureDependenceoftheSecondVirial Coefficient JunLiu,Da-ChuanYin*,Yun-ZhuGuo,Xi-KaiWang,Si-XiaoXie,Qin-QinLu,Yong-MingLiu KeyLaboratoryforSpaceBioscienceandBiotechnology,SchoolofLifeSciences,NorthwesternPolytechnicalUniversity,Xi’an,Shaanxi,People’sRepublicofChina Abstract Proteincrystalsusuallygrowatapreferabletemperaturewhichishowevernotknownforanewprotein.Thispaperreports a new approach for determination of favorable crystallization temperature, which can be adopted to facilitate the crystallization screening process. By taking advantage of the correlation between the temperature dependence of the secondvirialcoefficient(B22)andthesolubilityofprotein,wemeasuredthetemperaturedependenceofB22topredictthe temperaturedependenceofthesolubility.Usinginformationaboutsolubilityversustemperature,apreferredcrystallization temperature can be proposed. If B22 is a positive function of the temperature, a lower crystallization temperature is recommended; if B22 shows opposite behavior with respect to the temperature, a higher crystallization temperature is preferred.Otherwise,anytemperatureinthetestedrangecanbeused. Citation:LiuJ,YinD-C,GuoY-Z,WangX-K,XieS-X,etal.(2011)SelectingTemperatureforProteinCrystallizationScreensUsingtheTemperatureDependenceof theSecondVirialCoefficient.PLoSONE6(3):e17950.doi:10.1371/journal.pone.0017950 Editor:VladimirUversky,UniversityofSouthFloridaCollegeofMedicine,UnitedStatesofAmerica ReceivedDecember30,2010;AcceptedFebruary16,2011;PublishedMarch30,2011 Copyright:?2011Liuetal.Thisisanopen-accessarticledistributedunderthetermsoftheCreativeCommonsAttributionLicense,whichpermitsunrestricted use,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding:ThesestudieswerepartlyfundedbyGranromtheNationalNaturalScienceFoundationofChina(),Grant200806990011 from the Ph.D. Programs Foundation of the Ministry of Education of China (), Grant 2008AA1