Selection of Anti-Sulfadimidine Specific ScFvs from a Hybridoma Cell by Eukaryotic Ribosome Display 英文参考文献.docVIP

下载本文档

3
0
约3.49万字
约 8页
2017-05-13 发布于上海
举报
版权申诉

Selection of Anti-Sulfadimidine Specific ScFvs from a Hybridoma Cell by Eukaryotic Ribosome Display 英文参考文献.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Selection of Anti-Sulfadimidine Specific ScFvs from a Hybridoma Cell by Eukaryotic Ribosome Display 英文参考文献

SelectionofAnti-SulfadimidineSpecificScFvsfroma HybridomaCellbyEukaryoticRibosomeDisplay YonghuaQi1,2,CongmingWu1,SuxiaZhang1,ZhanhuiWang1,SiyangHuang1,LeiDai1 ,Shaochen Wang1,LiningXia1,3,KaiWen1,XingyuanCao1,YongningWu4,JianzhongShen1* 1NationalCenterforVeterinaryDrugSafetyEvaluation,CollegeofVeterinaryMedicine,ChinaAgriculturalUniversity,Beijing,China,2CollegeofAnimalScience,Henan InstituteofScienceandTechnology,Xixiang,China,3CollegeofVeterinaryMedicine,XinjiangAgriculturalUniversity,Urmuqi,China,4InstituteforNutritionandFood Safety,ChineseCenterforDiseaseControlandPrevention,Beijing,China Abstract Background:Ribosomedisplaytechnologyhasprovidedanalternativeplatformtechnologyforthedevelopmentofnovel low-costantibodybasedonevaluatingantibioticsderivedresiduesinfoodmatrixes. Methodology/Principal Findings: In our current studies, the single chain variable fragments (scFvs) were selected from hybridoma celllinesagainstsulfadimidine(SM2)byusingaribosomelibrary technology. ADNAlibrary ofscFvantibody fragmentswasconstructedforribosomedisplay,andthenmRNA–ribosome–antibody(MRA)complexeswereproducedby arabbitreticulocytelysatesystem.Thesyntheticsulfadimidine-ovalbumin(SM2-OVA)wasusedasanantigentopanMRA complexesandputativescFv-encodinggeneswererecoveredbyRT-PCRinsitufollowingeachpanning.Afterfourroundsof ribosome display, the expression vector pCANTAB5E containing the selected specific scFv DNA was constructed and transformedintoEscherichiacoliHB2151.Threepositiveclones(SAS14,SAS68andSAS71)werescreenedfrom100clones andhadhigherantibodyactivityandspecificitytoSM2byindirectELISA.ThethreespecificsolublescFvswereidentifiedto bethesamemolecularweight(approximately30kDa)byWestern-blottinganalysisusinganti-Etagantibodies,butthey haddifferentaminoacidssequencebysequenceanalysis. Conclusions/Significance: The selection of anti-SM2 specific scFv by in vitro ribosome display technology will have an importantsignificanceforthedevelopmentofnovelimmunodetectionstrategiesforresi