Selection of reference genes for quantitative real-time PCR expression studies in the apomictic and sexual grass Brachiaria brizantha 英文参考文献.docVIP

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Selection of reference genes for quantitative real-time PCR expression studies in the apomictic and sexual grass Brachiaria brizantha 英文参考文献.doc

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Selection of reference genes for quantitative real-time PCR expression studies in the apomictic and sexual grass Brachiaria brizantha 英文参考文献

BMC Plant Biology BioMedCentral Research article Open Access Selection of reference genes for quantitative real-time PCR expression studies in the apomictic and sexual grass Brachiaria brizantha érica Duarte Silveira1,2, Márcio Alves-Ferreira2, Larissa Arrais Guimar?es1, Felipe Rodrigues da Silva1 and Vera Tavares de Campos Carneiro*1 Address: 1Embrapa Genetic Resources and Biotechnology, Parque Esta??o Biológica, PqEB Av. W5 Norte (final) Caixa Postal 02372, Brasília, Brasil and 2Department of Genetics, Federal University of Rio de Janeiro Av. Prof. Rodolpho Paulo Rocco, s/n Prédio do CCS Instituto de Biologia, 2o Andar – Rio de Janeiro, RJ, Brasil Email: érica Duarte Silveira - ericads@.br; Márcio Alves-Ferreira - alvesfer@biologia.ufrj.br; Larissa Arrais Guimar?es - larissaarrais@.br; Felipe Rodrigues da Silva - felipes@cenargen.embrapa.br; Vera Tavares de Campos Carneiro* - vera@cenargen.embrapa.br * Corresponding author Published: 2 July 2009 Received: 25 November 2008 Accepted: 2 July 2009 BMC Plant Biology 2009, 9:84 doi:10.1186/1471-2229-9-84 This article is available from: /1471-2229/9/84 ? 2009 Silveira et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Brachiaria brizantha is an important forage grass. The occurrence of both apomictic and sexual reproduction within Brachiaria makes it an interesting system for understanding the molecular pathways involved in both modes of reproduction. Quantitative real time PCR (qRT-PCR) has emerged as an important technique to compare expression profile of target genes and, in order to obtain reliable results, it is important to have suitable reference genes. In this work, we evaluated eight potential reference genes for