Sensory Transduction Channel Subunits, tax-4 and tax-2, Modify Presynaptic Molecular Architecture in C. elegans 英文参考文献.docVIP

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Sensory Transduction Channel Subunits, tax-4 and tax-2, Modify Presynaptic Molecular Architecture in C. elegans 英文参考文献.doc

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Sensory Transduction Channel Subunits, tax-4 and tax-2, Modify Presynaptic Molecular Architecture in C. elegans 英文参考文献

SensoryTransductionChannelSubunits,tax-4andtax-2, ModifyPresynapticMolecularArchitectureinC.elegans AndrewB.Hellman1,KangShen1,2,3* 1DepartmentofBiology,StanfordUniversity,Stanford,California,UnitedStatesofAmerica,2HowardHughesMedicalInstitute,StanfordUniversity,Stanford,California, UnitedStatesofAmerica,3DepartmentofPathology,StanfordUniversity,Stanford,California,UnitedStatesofAmerica Abstract Duringdevelopment,neuralactivityisimportantforformingproperconnectionsinneuralnetworks.Theeffectofactivity on the gross morphology and synaptic strength of neurons has been well documented, but little is known about how activity affects different molecular components during development. Here, we examine the localization of four fluorescently-tagged presynaptic proteins, RAB-3, SNG-1/synaptogyrin, SYD-2/Liprin-a, and SAD-1/SAD kinase, in the C. elegans thermosensory neuron AFD. We show that tax-4 and tax-2, two genes that encode the cyclic nucleotide-gated channel necessary for sensory transduction in AFD, disrupt the localization of all four proteins. In wild-type animals, the synaptic vesicle (SV) markers RAB-3 and SNG-1 and the active zone markers SYD-2 and SAD-1 localize in a stereotyped, punctatepatternintheAFDaxon.Intax-4andtax-2mutants,SVandSYD-2punctaaremorenumerousandlessintense. Interestingly, SAD-1 puncta are also less intense but do not increase in number. The change in puncta number can be rescuedcell-autonomouslyinAFD.Theseresultssuggestthatsensorytransductiongenestax-4andtax-2arenecessaryfor theproperassemblyofpresynapses. Citation: Hellman AB, Shen K (2011) Sensory Transduction Channel Subunits, tax-4 and tax-2, Modify Presynaptic Molecular Architecture in C. elegans . PLoS ONE6(9):e24562.doi:10.1371/journal.pone.0024562 Editor:HiromuTanimoto,Max-Planck-Institutfu¨rNeurobiologie,Germany ReceivedDecember21,2010;AcceptedAugust15,2011;PublishedSeptember7,2011 Copyright: ? 2011 Hellman, Shen. This is an open-access article distributed under the terms of the Cr