Sequencing and de novo Analysis of Crassostrea angulata (Fujian Oyster) from 8 Different Developing Phases Using 454 GSFlx 英文参考文献.docVIP

下载本文档

11
0
约3.32万字
约 8页
2017-05-13 发布于上海
举报
版权申诉

Sequencing and de novo Analysis of Crassostrea angulata (Fujian Oyster) from 8 Different Developing Phases Using 454 GSFlx 英文参考文献.doc

1、本文档共8页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Sequencing and de novo Analysis of Crassostrea angulata (Fujian Oyster) from 8 Different Developing Phases Using 454 GSFlx 英文参考文献

SequencinganddenovoAnalysisofCrassostrea angulata(FujianOyster)from8DifferentDeveloping PhasesUsing454GSFlx JiQin1,2.,ZixiaHuang1,2.,JunChen1,2,QuanZou3,WeiweiYou1,2,CaihuanKe1,2 * 1StateKeyLaboratoryofMarineEnvironmentalScience,XiamenUniversity,Xiamen,China,2CollegeofOceanandEarthSciences,XiamenUniversity,Xiamen,China, 3CollegeofInformationScienceandTechnology,XiamenUniversity,Xiamen,China Abstract Research on the mechanism for early development of shellfish, such as body plan, shell formation, settlement and metamorphosisiscurrentlyanactiveresearchfield.However,studieswerestilllimitedandnotdeepenoughbecauseofthe lack of genomic resources such as genome or transcriptome sequences. In the present research, de novo transcriptome sequencingwasperformedforCrassostreaangulata,themosteconomicallyimportantculturedoysterspeciesinChina,at eightearlydevelopmentalstagesusingthe454sequencingtechnology.Atotalof555,215readswereproducedwithan average length of 309 nucleotides that were thenassembled into 10,462 contigs. As determined by GO annotation and KEGGpathwaymapping,functionalannotationoftheunigenesrecovereddiversebiologicalfunctionsandprocesses.Six uniquesequencesrelatedtosettlement,metamorphosisandgrowthweresubsequentlyanalyzedbyreal-timePCR.Given thelackofwholegenomeinformationforoysters,transcriptomeanddenovoanalysisofC.angulatafromtheeightdifferent developing phases will provide important and useful information on early development mechanism and help genetic breedingofshellfish. Citation:QinJ,HuangZ,ChenJ,ZouQ,YouW,etal.(2012)SequencinganddenovoAnalysisofCrassostreaangulata(FujianOyster)from8DifferentDeveloping PhasesUsing454GSFlx.PLoSONE7(8):e43653.doi:10.1371/journal.pone.0043653 Editor:TimothyRavasi,KingAbdullahUniversityofScienceandTechnology,SaudiArabia ReceivedApril15,2012;AcceptedJuly24,2012;PublishedAugust27,2012 Copyright:?2012Qinetal.Thisisanopen-accessarticledistributedunderthetermsoftheCreativeCommonsAttributionLicense,whichpermitsunrestricted use,distr