Sensory Experience Differentially Modulates the mRNA Expression of the Polysialyltransferases ST8SiaII and ST8SiaIV in Postnatal Mouse Visual Cortex 英文参考文献.docVIP

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Sensory Experience Differentially Modulates the mRNA Expression of the Polysialyltransferases ST8SiaII and ST8SiaIV in Postnatal Mouse Visual Cortex 英文参考文献.doc

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Sensory Experience Differentially Modulates the mRNA Expression of the Polysialyltransferases ST8SiaII and ST8SiaIV in Postnatal Mouse Visual Cortex 英文参考文献

SensoryExperienceDifferentiallyModulatesthemRNA ExpressionofthePolysialyltransferasesST8SiaIIand ST8SiaIVinPostnatalMouseVisualCortex Marie-ClaudeBe′langer,GraziellaDiCristo* CHUSainte-JustineandUniversite′ deMontre′al, Montreal,Quebec,Canada Abstract Polysialicacid(PSA)isauniquecarbohydratecomposedofalinearhomopolymerofa-2,8linkedsialicacid,andismainly attachedtothefifthimmunoglobulin-likedomainoftheneuralcelladhesionmolecule(NCAM)invertebrateneuralsystem. Inthebrain,PSAisexclusivelysynthesizedbythetwopolysialyltransferasesST8SiaII(alsoknownasSTX)andST8SiaIV(also knownasPST).BymodulatingadhesivepropertyofNCAM,PSAplaysacriticalroleinseveralneuraldevelopmentprocesses such as cell migration, neurite outgrowth, axon pathfinding, synaptogenesis and activity-dependent plasticity. The expressionofPSAistemporallyandspatiallyregulatedduringneuraldevelopmentandatightregulationofPSAexpression isessentialtoitsbiologicalfunction.Inmousevisualcortex,PSAisdownregulatedfollowingeyeopeninganditsdecrease allows the maturation of GABAergic synapses and the opening of the critical period for ocular dominance plasticity. Relatively little is known about how PSA levels are regulated by sensory experience and neuronal activity. Here, we demonstratethatwhilebothST8SiaIIandST8SiaIVmRNAlevelsdecreasearoundthetimeofeyeopeninginmousevisual cortex,onlyST8SiaIImRNAlevelreductionisregulatedbysensoryexperience.Usinganorganotypicculturesystemfrom mousevisualcortex,wefurthershowthatST8SiaIIgeneexpressionisregulatedbyspikingactivityandNMDA-mediated excitation. Further, we show that both ST8SiaII and ST8SiaIV mRNA levels are positively regulated by PKC-mediated signaling.Therefore,sensoryexperience-dependentST8SiaIIgeneexpressionregulatesPSAlevelsinpostnatalvisualcortex, thusactingasmolecularlinkbetweenvisualactivityandPSAexpression. Citation:Be′langerM-C,DiCristoG(2011)SensoryExperienceDifferentiallyModulatesthemRNAExpressionofthePolysialyltransferasesST8SiaIIandST8SiaIVin PostnatalMouseVisualCo