Shapes of Differential Pulse Voltammograms and Level of Metallothionein at Different Animal Species 英文参考文献.docVIP

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Shapes of Differential Pulse Voltammograms and Level of Metallothionein at Different Animal Species 英文参考文献.doc

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Shapes of Differential Pulse Voltammograms and Level of Metallothionein at Different Animal Species 英文参考文献

Sensors 2007, 7, 2419-2429 sensors ISSN 1424-8220 ? 2007 by MDPI /sensors Full Research Paper Shapes of Differential Pulse Voltammograms and Level of Metallothionein at Different Animal Species Vojtech Adam 1,2, Miroslava Beklova 3, Jiri Pikula 3, Jaromir Hubalek 4, Libuse Trnkova 4 and Rene Kizek 1,* 1 Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic 2 Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic 3 Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, CZ-612 42 Brno, Czech Republic 4 Department of Microelectronics, Faculty of Electrical Engineering and Communication, Brno University of Technology, Udolni 53, CZ-602 00 Brno, Czech Republic 5 Department of Chemistry, Faculty of Science, Masaryk University, Kotlarska 37, CZ-611 37 Brno, Czech Republic * Author to whom correspondence should be addressed. E-mail:kizek@sci.muni.cz Received: 1 October 2007 / Accepted: 17 October 2007 / Published: 19 October 2007 Abstract: Metallothioneins play a key role in maintaining homeostasis of essential metals and in protecting of cells against metal toxicity as well as oxidative damaging. Excepting humans, blood levels of metallothionein have not yet been reported from any animal species. Blood plasma samples of 9 animal species were analysed by the adsorptive transfer stripping technique to obtain species specific voltammograms. Quite distinct records were obtained from the Takin (Budorcas taxicolor), while other interesting records were observed in samples from the European Bison (Bison bonasus bonasus) and the Red-eared Slider (Trachemys scripta elegans). To quantify metallothionein the catalytic peak Cat2 w