Sheathless Size-Based Acoustic Particle Separation 英文参考文献.docVIP

Sensors 2012, 12, 905-922; doi:10.3390/s120100905 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article Sheathless Size-Based Acoustic Particle Separation Rasim Guldiken 1,*, Myeong Chan Jo 1, Nathan D. Gallant 1, Utkan Demirci 2,3 and Jiang Zhe 4,* 1 Department of Mechanical Engineering, University of South Florida, Tampa, FL 33620, USA; E-Mails: mjo@ (M.C.J.); ngallant@ (N.D.G.) 2 Center for Biomedical Engineering, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA 02115, USA; E-Mail: utkan@ 3 Harvard-MIT Health Sciences and Technology, Cambridge, MA 02139, USA 4 Department of Mechanical Engineering, University of Akron, Akron, OH 44325, USA * Authors to whom correspondence should be addressed; E-Mails: guldiken@ (R.G.); jzhe@ (J.Z.); Tel.: +1-813-974-5628 (R.G.); Fax: +1-813-974-3539 (R.G.); Tel.: +1-330-972-7737 (J.Z.); Fax: +1-330-972-6027 (J.Z.). Received: 23 November 2011; in revised form: 4 January 2012 / Accepted: 14 January 2012 / Published: 16 January 2012 Abstract: Particle separation is of great interest in many biological and biomedical applications. Flow-based methods have been used to sort particles and cells. However, the main challenge with flow based particle separation systems is the need for a sheath flow for successful operation. Existence of the sheath liquid dilutes the analyte, necessitates precise flow control between sample and sheath flow, requires a complicated design to create sheath flow and separation efficiency depends on the sheath liquid composition. In this paper, we present a microfluidic platform for sheathless particle separation using standing surface acoustic waves. In this platform, particles are first lined up at the center of the channel without introducing any external sheath flow. The particles are then entered into the second stage where particles are driven towards the off-center pressure nodes for size bas