SFE-CO2 Extract from Typhonium giganteum Engl. Tubers, Induces Apoptosis in Human Hepatoma SMMC-7721 Cells Involvement of a ROS-Mediated Mitochondrial Pathway 英文参考文献.docVIP

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SFE-CO2 Extract from Typhonium giganteum Engl. Tubers, Induces Apoptosis in Human Hepatoma SMMC-7721 Cells Involvement of a ROS-Mediated Mitochondrial Pathway 英文参考文献.doc

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SFE-CO2 Extract from Typhonium giganteum Engl. Tubers, Induces Apoptosis in Human Hepatoma SMMC-7721 Cells Involvement of a ROS-Mediated Mitochondrial Pathway 英文参考文献

Molecules 2011, 16, 8228-8243; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article SFE-CO2 Extract from Typhonium giganteum Engl. Tubers, Induces Apoptosis in Human Hepatoma SMMC-7721 Cells Involvement of a ROS-Mediated Mitochondrial Pathway Qingyong Li 1,2,*, Chunfei Jiang 1,2, Yuangang Zu 1,2, Zhen Song 1,2, Baoyou Zhang 1,2, Xiangdong Meng 1,2, Wei Qiu 1,2 and Li Zhang 1,2 1 Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China; E-Mail: jiangchunfei2009@126.com (C.J.) 2 Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, China; E-Mails: zygorl@ (Y.Z.); songzhen55@163.com (Z.S.); 605764896@ (B.Z.); 289122789@ (X.M.); qiuwei3042@126.com (W.Q.); ally521@126.com (L.Z.) * Author to whom correspondence should be addressed; E-Mail: li_qingyong@126.com; Tel.: +86-451 Fax: +86-451 Received: 9 August 2011; in revised form: 20 September 2011 / Accepted: 22 September 2011 / Published: 28 September 2011 Abstract: Typhonium giganteum Engl. (BaiFuzi) is one of the herbs commonly used in traditional Chinese medicine against cancer. In our previous studies, 37 compounds were identified the SFE-CO2 (supercritical fluid extraction with CO2) extract by GC-MS, including the four major components [β-sitosterol (40.22%), campesterol (18.45%), n-hexadecanoic acid (9.52%) and (Z,Z)-9,12-octadecadienoic acid (8.15%)]. The anti-cancer mechanisms of the SFE-CO2 extract from T. giganteum Engl. tubers have not been reported as yet. In this paper, the molecular mechanisms of the SFE-CO2 extract-mediated apoptosis in SMMC-7721 cells were further examined. SFE-CO2 extract inhibited the growth of SMMC-7721 cells in a time- and dose-dependent manner, arrested the cell cycle in the S phase and G2/M phase, and induced apoptosis. In addition, r