Significance of Coronavirus Mutants in Feces and Diseased Tissues of Cats Suffering from Feline Infectious Peritonitis 英文参考文献.docVIP

Viruses 2009, 1, 166-184; doi:10.3390/v1020166 OPEN ACCESS viruses ISSN 1999-4915 /journal/viruses Article Significance of Coronavirus Mutants in Feces and Diseased Tissues of Cats Suffering from Feline Infectious Peritonitis Niels C. Pedersen 1,*, Hongwei Liu 1, Kimberly A. Dodd 2 and Patricia A. Pesavento 3 1 Center for Companion Animal Health, School of Veterinary Medicine, University of California, One Shields Avenue, Davis, CA, 95616, USA; E-mail: hwlliu@ (H.L.) 2 School of Veterinary Medicine, University of California, One Shields Avenue, Davis, CA, 95616, USA; E-Mail: kadodd@ (K.A.D.) 3 Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA, 95616, USA; E-Mail: papesavento@ (P.A.P.) * Author to whom correspondence should be addressed; E-Mail: ncpedersen@; Tel.: +1-530-752-7402; Fax: +1-530-752-7701. Received: 17 July 2009; in revised form: 10 August 2009 / Accepted: 11 August 2009 / Published: 26 August 2009 Abstract: The internal FECV→FIPV mutation theory and three of its correlates were tested in four sibs/half-sib kittens, a healthy contact cat, and in four unrelated cats that died of FIP at geographically disparate regions. Coronavirus from feces and extraintestinal FIP lesions from the same cat were always 99% related in accessory and structural gene sequences. SNPs and deletions causing a truncation of the 3c gene product were found in almost all isolates from the diseased tissues of the eight cats suffering from FIP, whereas most, but not all fecal isolates from these same cats had intact 3c genes. Other accessory and structural genes appeared normal in both fecal and lesional viruses. Deliterious mutations in the 3c gene were unique to each cat, indicating that they did not originate in one cat and were subsequently passed horizontally to the others. Compartmentalization of the parental and mutant forms was not absolute;