Small chloroplast-targeted DnaJ proteins are involved in optimization of photosynthetic reactions in Arabidopsis thaliana 英文参考文献.docVIP

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Small chloroplast-targeted DnaJ proteins are involved in optimization of photosynthetic reactions in Arabidopsis thaliana 英文参考文献.doc

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Small chloroplast-targeted DnaJ proteins are involved in optimization of photosynthetic reactions in Arabidopsis thaliana 英文参考文献

Chenetal.BMCPlantBiology2010,10:43 /1471-2229/10/43 RESEARCH ARTICLE OpenAccess Smallchloroplast-targetedDnaJproteinsare involvedinoptimizationofphotosynthetic reactionsinArabidopsisthaliana Kun-MingChen2,MaijaHolmstr?m1,WuttinunRaksajit1,MarjaanaSuorsa1,MirvaPiippo1,Eva-MariAro1* Abstract Background:DnaJproteinsparticipateinmanymetabolicpathwaysthroughdynamicinteractionswithvarious componentsoftheseprocesses.Theroleofthreesmallchloroplast-targetedDnaJproteins,AtJ8(At1g80920), AtJ11(At4g36040)andAtJ20(At4g13830),wasinvestigatedhereusingknock-outmutantsofArabidopsisthaliana. Photochemicalefficiency,capacityofCO2assimilation,stabilizationofPhotosystem(PS)IIdimersand supercomplexesunderhighlightillumination,energydistributionbetweenPSIandPSIIandphosphorylationof PSII-LHCIIproteins,globalgeneexpressionprofilesandoxidativestressresponsesoftheseDnaJmutantswere analyzed. Results:Knockoutofoneoftheseproteinscausedaseriesofeventsincludingadecreaseinphotosynthetic efficiency,destabilizationofPSIIcomplexesandlossofcontrolforbalancingtheredoxreactionsinchloroplasts. DataobtainedwithDNAmicroarrayanalysisdemonstratedthatthelackofoneoftheseDnaJproteinstriggersa globalstressresponseandthereforeconferstheplantsgreatertolerancetooxidativestressinducedbyhighlight ormethylviologentreatments.Expressionofasetofgenesencodingenzymesthatdetoxifyreactiveoxygen species(ROS)aswellasanumberofstress-relatedtranscriptionfactorsbehavedinthemutantsatgrowthlight similarlytothatwhenwild-type(WT)plantsweretransferredtohighlight.Alsoasetofgenesrelatedtoredox regulationwereupregulatedinthemutants.Ontheotherhand,althoughthethreeDnaJproteinsresidein chloroplasts,theexpressionofmostgenesencodingthylakoidmembraneproteinswasnotchangedinthe mutants. Conclusion:ItisproposedthatthetoleranceoftheDnaJproteinknockoutplantstooxidativestressoccursatthe expenseoftheflexibilityofphotosyntheticreactions.Despitethefactthattheeffectsoftheindividualprotein knockoutontheresponseofplantstohighlighttreatmentarequitesimila