Surface Plasmon Spectroscopic Detection of Saxitoxin 英文参考文献.docVIP

Sensors 2007, 7, 1216-1223 sensors ISSN 1424-8220 ? 2007 by MDPI /sensors Communication Surface Plasmon Spectroscopic Detection of Saxitoxin Hongxia Chen 1, Youn Sook Kim 2, Sam-Rok Keum 3, Sung-Hoon Kim 4, Heung-Jin Choi 4, Jaebeom Lee 2, Won Gun An 2 and Kwangnak Koh 2, * 1 College of Pharmacy, Pusan National University, Pusan 609-735, Korea 2 College of Nanoscience and Nanotechnology, Pusan National University, Pusan 609-735, Korea 3 College of Science and Technology, Korea University, Choong-nam 339-700, Korea 4 College of Engineering, Kyungpook National University, Taegu 702-701, Korea * Author to whom correspondence should be addressed; E-mail: koh@pusan.ac.kr Received: 18 June 2007 / Accepted: 13 July 2007 / Published: 16 July 2007 Abstract: For the surface-optoelectronic study of Saxitoxin sensing, we fabricated self- assembled calix[4]arene derivative monolayers as the recognition-functional interfaces on a gold surface. An interaction study between Saxitoxin and calix[4]arene derivative monolayers were performed using surface plasmon resonance (SPR) spectroscopy. Among three calix[4]arene derivatives, calix[4]arene crown ether SAM showed the highest sensitivity to Saxitoxin. The detection limit of this system is three orders of magnitude lower than that of the mouse bioassay which is the current benchmark for Saxitoxin detection. Keywords: Saxitoxin, surface plasmon resonance, calixarene, self-assembled monolayer (SAM). 1. Introduction Harmful algal booms (red tides) produce a wide variety of secondary metabolites, but Saxitoxin (STX) is virtually isolated among them in being capable of causing human mortality by consumption of tainted shellfish. STX and its congeners are known as paralytic shellfish poisons (PSPs). Currently, governments of many countries monitor shellfish beds for the presence of saxitoxin by using one of several tests, the most reliable test is mouse