Supermolecular Interaction of Ferrocenium with Yeast DNA and Application in Electrochemical Sensing for Hybridization Recognition of Yeast DNA 英文参考文献.docVIP

Sensors 2004, 4, 71-83 sensors ISSN 1424-8220 ? 2004 by MDPI /sensors Supermolecular Interaction of Ferrocenium with Yeast DNA and Application in Electrochemical Sensing for Hybridization Recognition of Yeast DNA Huangxian Ju*, Baofen Ye and Jiayin Gu Department of Chemistry, State Key Laboratory of Coordination Chemistry, Institute of Chemical Biology, Nanjing University, Nanjing 210093, China, Fax: +86-25-3593593, Tel: +86-25-3593593 * Author to whom correspondence should be addressed. E-mail: hxju@ Received: 30 April 2004 / Accepted: 30 May 2004 / Published: 20 July 2004 Abstract: The supermolecular interactions of single-stranded yeast DNA (ssDNA) and + ) both in solution and at the double-stranded DNA (dsDNA) with ferrocenium (Fc electrode/solution interface were studied with UV spectroscopy and electrochemical method. The interaction of covalently immobilized dsDNA with Fc resulted in a change of the electrode behavior from diffusion-controlled to surface-controlled and an increase in the current response of Fc reduction, which has been used to develop a novel electrochemical yeast DNA sensor for hybridization recognition of immobilized yeast ssDNA to its complementary ssDNA (cDNA). The adsorption constants of Fc on ssDNA and dsDNA modified gold electrode surface were (3.38±0.04)×10 and (2.02±0.02)×10 respectively, indicating a higher affinity of dsDNA to Fc ion strength showed that the interaction mode between Fc + + + 3 M -1 M , 4 -1 . UV spectra and the influence of and dsDNA in solution might be a + + groove binding. The interaction between Fc and immobilized DNA was mainly an + electrostatic model. Keywords: DNA Biosensors, supermolecular interaction, yeast DNA, ferrocenium, hybridization recognition.  Sensors 2004, 4 72 Introduction DNA biosensors have become an important research ar