Transcript and proteomic analysis of developing white lupin (Lupinus albus L.) roots 英文参考文献.docVIP

BMC Plant Biology BioMedCentral Research article Open Access Transcript and proteomic analysis of developing white lupin (Lupinus albus L.) roots Li Tian*?1,2, Gregory J Peel?1, Zhentian Lei1, Naveed Aziz1,3, Xinbin Dai1, Ji He1, Bonnie Watson1, Patrick X Zhao1, Lloyd W Sumner1 and Richard A Dixon1 Address: 1Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK 73401, USA, 2Department of Plant Sciences, University of California, Davis, Davis, CA 95616, USA and 3CNAP, Department of Biology, University of York, York, YO 10 5YW, UK Email: Li Tian* - ltian@; Gregory J Peel - gjpeel@; Zhentian Lei - zlei@; Naveed Aziz - na9@york.ac.uk; Xinbin Dai - xdai@; Ji He - jhe@; Bonnie Watson - bowatson@; Patrick X Zhao - pzhao@; Lloyd W Sumner - lwsumner@; Richard A Dixon - radixon@ * Corresponding author ?Equal contributors Published: 5 January 2009 Received: 16 September 2008 Accepted: 5 January 2009 BMC Plant Biology 2009, 9:1 doi:10.1186/1471-2229-9-1 This article is available from: /1471-2229/9/1 ? 2009 Tian et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: White lupin (Lupinus albus L.) roots efficiently take up and accumulate (heavy) metals, adapt to phosphate deficiency by forming cluster roots, and secrete antimicrobial prenylated isoflavones during development. Genomic and proteomic approaches were applied to identify candidate genes and proteins involved in antimicrobial defense and (heavy) metal uptake and translocation. Results: A cDNA library was constructed from roots of white lupin seedlings. Eight thousand clones were randomly sequenced and assembled into 2,455 unigenes, which were annotated based on homologous matches in the NCBInr prot