Triterpene Composition and Bioactivities of Centella asiatica 英文参考文献.docVIP

Molecules 2011, 16, 1310-1322; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Triterpene Composition and Bioactivities of Centella asiatica Puziah Hashim 1,*, Hamidah Sidek 2, Mohd Helme M. Helan 2, Aidawati Sabery 2, Uma Devi Palanisamy 3 and Mohd Ilham 4 1 Halal Products Research Institute, Universiti Putra Malaysia, Putra Infoport, 43400 UPM Serdang, Selangor, Malaysia 2 Industrial Biotechnology Research Centre, SIRIM Berhad, 1 Persiaran Dato’ Menteri, 40911 Shah Alam, Selangor, Malaysia 3 School of Medicine and Health Sciences, Monash University Sunway Campus, Jalan Lagoon Selatan, 46150 Bandar Sunway, Selangor, Malaysia 4 Forest Research Institute Malaysia, 52109, Kepong, Selangor, Malaysia * Author to whom correspondence should be addressed; E-Mail: puziahashim@; Tel.: 603-8941 7344/7434/7971; Fax: 603-8943 9745. Received: 25 November 2010; in revised form: 4 January 2011 / Accepted: 13 January 2011 / Published: 28 January 2011 Abstract: Leaves of Centella asiatica (Centella) were analysed for their triterpene composition and bioactivity such as collagen enhancement, antioxidant, anticellulite and UV protection capacity properties. Triterpenes of Centella were measured using HPLC- PAD on an Excil ODS 5 μm (C18) column for the simultaneous determination of asiatic acid, madecassic acid, asiaticoside and madecassoside. Centella was found to contain significant amounts of madecassoside (3.10 ± 4.58 mg/mL) and asiaticoside (1.97 ± 2.65 mg/mL), but was low in asiatic and madecassic acid. The highest collagen synthesis was found at 50 mg/mL of Centella extracts. The antioxidant activity of Centella (84%) was compared to grape seed extract (83%) and Vitamin C (88%). Its lipolytic activity was observed by the release of glycerol (115.9 μmol/L) at 0.02% concentration. Centella extracts exhibited similar UV protection