Use of Fe3O4 Nanoparticles for Enhancement of Biosensor Response to the Herbicide 2,4-Dichlorophenoxyacetic Acid 英文参考文献.docVIP

Sensors 2008, 8, 5775-5791; DOI: 10.3390/s8095775 OPEN ACCESS sensors ISSN 1424-8220 /sensors Article Use of Fe3O4 Nanoparticles for Enhancement of Biosensor Response to the Herbicide 2,4-Dichlorophenoxyacetic Acid Kee-Shyuan Loh 1, Yook Heng Lee 1,*, Ahmad Musa 1, Abdul Aziz Salmah 2 and Ishak Zamri2 1 School of Chemical Sciences and Food Technology, Faculty of Science and Technology, University Kebangsaan Malaysia, Bangi, 43000, Selangor, Malaysia; E-mails: keeshyuan@; andong@ukm.my 2 Biotechnology Research Centre, Malaysian Agriculture Research Development Institute (MARDI), P.O. BOX 12301, GPO Kuala Lumpur, Malaysia; E-mails: salmahaa@.my; zamri@.my * Author to whom correspondence should be addressed; E-mails: yhl1000@ukm.my; leeyookheng@yahoo.co.uk; Tel.: +603 Fax: +603 Received: 29 July 2008; in revised form: 30 August 2008 / Accepted: 10 September 2008 / Published: 18 September 2008 Abstract: Magnetic nanoparticles of Fe3O4 were synthesized and characterized using transmission electron microscopy and X-ray diffraction. The Fe3O4 nanoparticles were found to have an average diameter of 5.48 ±1.37 nm. An electrochemical biosensor based on immobilized alkaline phosphatase (ALP) and Fe3O4 nanoparticles was studied. The amperometric biosensor was based on the reaction of ALP with the substrate ascorbic acid 2-phosphate (AA2P). The incorporation of the Fe3O4 nanoparticles together with ALP into a sol gel/chitosan biosensor membrane has led to the enhancement of the biosensor response, with an improved linear response range to the substrate AA2P (5-120 μM) and increased sensitivity. Using the inhibition property of the ALP, the biosensor was applied to the determination of the herbicide 2,4-dichlorophenoxyacetic acid (2,4- D). The use of Fe3O4 nanoparticles gives a two-fold improvement in the sensitivity towards 2,4-D, with a linear response range of 0.