Accurate proteome-wide protein quantification from high-resolution 15N mass spectra.docVIP

Khanetal.GenomeBiology2011,12:R122 /2012/12/12/R122 METHOD OpenAccess Accurateproteome-wideproteinquantification 15 fromhigh-resolution Nmassspectra ZiaKhan1,2,3,SasanAmini2,4,5?,JoshuaSBloom2,4,CristianRuse6,AmyACaudy2,7,LeonidKruglyak2,8,9, MonaSingh1,2,DavidHPerlman2,4,10 andSaeedTavazoie2,4,11* Abstract Inquantitativemassspectrometry-basedproteomics,themetabolicincorporationofasinglesourceof 15 nitrogenhasmanyadvantagesoverusingstableisotope-labeledaminoacids.However,thelackofarobust computationalframeworkforanalyzingtheresultingspectrahasimpededwideuseofthisapproach.Wehave