Purification of rabbit thymus HMGN2 Identification.docVIP

Purification of rabbit thymus HMGN2 Identification [Abstract] Objective: Identification of purified rabbit non-high mobility group protein N2 (High mobility group chromosomal protein N2, HMGN2). Methods: Using liquid nitrogen grinding and 5% perchloric acid obtained from fresh rabbit thymus tissue acid-soluble extract, by high performance liquid chromatography (RP HPLC isolated HMGN2, after SDS polyacrylamide gel electrophoresis and Western blotting for molecular weight determination of HMGN2 and specificity of identification. and the use of agarose diffusion test its bactericidal antibacterial activity were identified. Results: Using this method high purity purified rabbit HMGN2. 54 080 clinical isolates of Escherichia coli, Escherichia coli ATCC25922 significant bactericidal activity. Conclusion: The simple separation of high purity HMGN2 approach. [Keywords:] rabbit thymus, HMGN2, separation Non-high mobility group protein (High mobility group chromosomal protein, HMG) is the nucleus of vertebrates and invertebrates the most abundant family of non-histone proteins [9], a member of this family exists in higher eukaryotes currently divided into three sub HMG protein family, namely HMGA, HMGB and HMGN HMGN.HMGN2 as a member of subfamily, the gene located on chromosome 1q35, relative molecular mass 9.2KD, a total of 90 amino acids. It has been that, HMGN2 with specific parts of the chromosome is an essential part of the chromosome fibers [2,3], involved in DNA replication and transcription [1], and the transcriptional activity of genes [5], to solve the dense chromosomes folded structure which is conducive to start the replication and transcription [6]. HMGN2 first discovered in our laboratory has obvious antibacterial activity [4]. HMGN2 to further study the biological function, we used agar diffusion assay for endogenous antimicrobial method of antimicrobial peptides, purification of rabbit thymus HMGN2. 1 Materials and methods 1.1 Materials Ingre