Real-time fluorescence quantitative PCR experimental study.docVIP

Real-time fluorescence quantitative PCR experimental study Abstract Real-time quantitative PCR technology is in general PCR technique developed on the basis of qualitative nucleic acid quantification technology, has been widely applied in different fields of study. Discusses the quantitative PCR technology, the basic principles, main types and quantitative experimental methods, experimental conditions of control, influence factors and the optimization of experimental outcomes. Keywords: fluorescence quantitative PCR technology; principle; main types; quantitative methods; Optimization Abstract Real-time fluorescent quantitative PCR is a new kind of nucleic acid quantitative technique, which develops in the PCR qualitative technique base. It has been widely used in different research fields. In the paper, the experiment principles, the main types and quantitative methods of this technology were described. The control of experimental conditions, affecting factors and optimization of experimental results were discussed. Keywords: real-time fluorescent quantitative PCR; principle; main types; quantitative method; optimizing Real-time quantitative PCR technology in 1996, Applied Biosystems launched by the United States, the PCR technique not only to achieve the leap from qualitative to quantitative, but also has high sensitivity, specificity, and reliability, multiple responses to achieve high degree of automation, no pollution, and accuracy with real-time characteristics [1]. Currently, the technology has been widely used in molecular biology, medicine and other disciplines and basic research, especially in the modern agricultural genetically modified crops, qualitative detection, strain identification, GM Detection of ingredients play an important role [2]. 1 the principle of quantitative PCR technology Quantitative PCR, PCR reaction system was added in the fluorophore, the fluorescence signal accumulated by the PCR process real-time monit