分子生物学（张海红）CHAPTER 12.pptVIP

Function of 5′cap Protection from degradation Increased translational efficiency Transport to cytoplasm Splicing of first intron Function of poly(A) tail Increased mRNA stability Increased translational efficiency Splicing of last intron The “cap”: a methylated guanine joined to the RNA transcript by a 5’-5’ linkage The linkage contains 3 phosphates 3 sequential enzymatic reactions Occurs early RNA processing 15’ end capping Splicing: joining the protein coding sequences Dephosphorylation of Ser5 within the CTD tail leads to dissociation of capping machinery Further phosphorylation of Ser