鸡胚地龙膏对急性软组织损伤模型组织病理学及细胞因子的影响.docVIP

鸡胚地龙膏对急性软组织损伤模型组织病理学及细胞因子的影响 　　 【摘要】 目的 探讨鸡胚地龙膏对动物急性软组织损伤的治疗作用及其机制。 　　方法 105只新西兰大白兔,随机选出21只为正常对照组，剩余84只新西兰白兔制作左后腿部急性软组织损伤模型,造模成功后随机分为模型对照组、阳性药物组(奇正消痛膏组),鸡胚低剂量组和鸡胚高剂量组,每组21只动物。模型对照组不作处理，自然恢复, 阳性药物组采用奇正消痛膏治疗(1 g/kg)，鸡胚低剂量组和鸡胚高剂量组采用鸡胚地龙膏治疗，药物剂量分别为0.15 g/kg和0.75 g/kg。动态测定新西兰大白兔急性软组织损伤模型组织病理学评分、血浆黏度、组织学及形态计量学指标,同时测定血浆及组织液中IL-1β水平的含量变化。结果 鸡胚地龙膏对急性软组织损伤动物模型有良好的恢复作用, 鸡胚高剂量组在病情程度评估、组织病理学、血浆及损伤局部组织中白细胞介素-1β的含量下降方面均优于模型组 (P＜0.05或0.01)。结论 鸡胚地龙膏外用治疗新西兰大白兔急性软组织损伤, 能明显降低血液黏度、改善微循环和降低炎症介质IL-1β释放，促进损伤组织的修复。 　　 【关键词】 鸡胚地龙膏；急性软组织损伤；组织病理学；血浆黏度；白细胞介素-1 　　 文章编号：1003-1383(2011)06-0687-04 中图分类号：R 361+.2 文献标识码：A 　　doi:10.3969/j.issn.1003-1383.2011.06.001 　　 　　 Effects of Jipei Ointment on Histopathology and Cytokineof Model of Acute Soft Tissue Injur 　　SHEN Zhenhua??1,YANG Li??2, LUO Guozhong??1,YU Feng,LUO Haiyan??2,HUANG Yuena??3,HU Jianshan??4 　　(1.Pathology Department, Hospital of Tradition Chinese Medicine of Qiannan 　　AutonomousPrefecture,Duyun 558000；2.Teaching and research room of 　　Histoembryology, Qiannan Medical College for Nationalities, Duyun 558001; 　　3.Clinical laboratory, Hospital of Tradition Chinese Medicine ofQiannan Autonomous Prefecture, Duyun 558000; 4.Orthopedics Department, Hospital of TraditionChinese Medicine of Qiannan Autonomous Prefecture, Duyun 558000,Guizhou,China) 　　 【Abstract】 Objective To discuss the therapeutic effect and the mechanism of Jipei ointment on animals with acute soft tissue injury. 　　Methods Among 105 New Zealand white rabbits, 21 were selected randomly as the normal control group.The other (84) were modeled as acute soft tissue injury to their left hind legs.After the success of modeling,these rabbits with acute soft tissue injury were divided into four groups:the model control group,the positive drug group(the Qizheng pain relieving ointment group),the low dose Jipei ointment group and the high dose Jipei ointment group,with 21 rabbits in each group.The model control group was not treated by medication and they were left recove