Evaluation of the Bioactivity of Novel Spiroisoxazoline TypeCompounds against Normal and Cancer Cell Lines.docVIP

Molecules 2010, 15, 9340-9353; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Evaluation of the Bioactivity of Novel Spiroisoxazoline Type Compounds against Normal and Cancer Cell Lines Nigar Najim 1,*, Yaser Bathich 2, Mazatulikhma Mat Zain 1, Ahmad Sazali Hamzah 2 and Zurina Shaameri 2 1 Tissue Culture Research Laboratory, Centre of Synthesis and Chemical Biology, Institute of Science, University Technology MARA, 40450 Shah Alam, Malaysia; E-Mail: maizatul@.my (M.M.Z.) 2 Organic Synthesis Research Laboratory, Centre of Synthesis and Chemical Biology, Institute of Science, University Technology MARA, 40450 Shah Alam, Malaysia; E-Mails: bathich@ (Y.B.); asazali@.my (A.S.H.); zurina@.my (Z.S.) * Author to whom correspondence should be addressed; E-Mail: nigar@.my; Tel.: +603 5544 4482; Fax: +603 5544 3870. Received: 5 October 2010; in revised form: 3 November 2010 / Accepted: 2 December 2010 / Published: 17 December 2010 Abstract: The aim of this study was to investigate the in vitro cellular activity of novel spiroisoxazoline type compounds against normal and cancer cell lines from lung tissue (Hs888Lu), neuron-phenotypic cells (SH-SY5Y), neuroblastoma (SH-SY5Y), human histiocytic lymphoma (U937), lung cancer (A549), and leukaemia (HL-60). Our bioassay program revealed that the spiroisoxazoline type compounds show cytotoxicity only in lymphoma cell lines, which is in contrast with the pyrrolidine precursor of these spiroisoxazoline compounds, where significant cytotoxicity is seen in all normal and cancer cell lines. These data suggest a tumour-specific mechanism of action. In addition these data also show that spiroisoxazoline compounds are non-toxic in the human neuron- phenotypic neuroblastoma SH-SY5Y cell line, and furthermore that they might protect cells from neurodegenerative disease. Keywords: pyrrolidine,