脂溶性红松籽壳色素的初步分离与纯化.pdfVIP

安徽农业科学，Joumal of Anhui Agri. Sci. 2011 ,39 (1 5) :8940 -8945 ,8978 责任编辑陈玉敏责任校对卢瑶 脂溶性红松籽壳色素的初步分离与纯化 文攀1 ，王振宇1 气夏祥慧3 (1 琼州学院生物科学与技术学院，海南五指山 57剧;2 哈尔滨工业大学食品科学研究院，黑龙江哈尔 滨 15创则1 ;3. 牡丹江医学院临床医学院，黑龙江牡丹江 157011) 摘要 [目的]为脂溶性红松种壳色素化学成分的进一步研究提供理论依据。[方法]以硅胶为吸附剂，通过溶剂梯度洗脱对脂溶性红 松种壳色素进行分离与纯化，探索利用硅胶柱层析对脂溶性红松松籽壳色素的分离效果。[结果]951J毛乙醇为脂溶性红松松籽壳色素的 最佳提取剂。对月旨溶性红松松籽壳色素进行初步分离与纯化，得到 26 纽洗脱物，主要在波长 250 和270 nm 处出现强吸收，可见光区无 明显吸收，由此推测这些物质主要是黄嗣类化合物。[结论]硅胶拉色谱法对脂溶性红松种先色素具有较好的分离效采。 关键词 红松;红松松籽壳;色素;分离与纯化 中国分类每 F132 文献标识码 A 文章编号 0517 -6611 (2011) 15 -08940-06 Preliminary Separation and Purification of Liposoluble Pinenut Shell Pigments from Pinus koraiensis WEN Pan et al (Department of Life Science , Qiongzhou University , Wuzhi8han ,Hainan 5722∞) Abstract [ Objective] The research aimed to provide theoretical basis for further study on the chemical components of liposoluble pinenut shell pi伊ents from Pinus koraiensis. [Method] With si1ica gel as sorbent , the liposoluble pinenut shell pi伊ents from P. koraiensis was sepa- rated and purified by using solvent gradient elution. The separation effects of liposoluble pinenut shell pigments from P. koraiensis by using si1- ica gel column chromatography were discussed. [Result ]币le best extraction solvent for liposoluble pinenut shell pigments from P. kora伦nsis was 95 % ethanol. Mter preliminary 回paration and purification of liposoluble pinenut shell pigments from P. koraiens白， 26 eluting materials were obtained. Strong absorption appeared at the 叹avelength of 250 and 270 nm and no significant absorption was 胆en in visible light area. It W国 concluded that these substances we陀 mainly flavonoids compounds. [Conclusion] Better separation effects of lip怖。luble pinenut shell pigments from P. koraiensis were obtained by using silica gel column chromatography. Key words Pinus koraien.s is; Pinenut shell of Pinus koraie