自然流产患者蜕膜组织疾病特异蛋白的初步筛选与鉴定.pdfVIP

重庆医学 2010 年 11 月第 39 卷第 21 期 2861 ·论著· 自然流产患者蜕膜组织疾病特异蛋白的初步筛选与鉴定铃 陈倚，谭彬，王应雄，黎刚，胡建刚，余秋波A (重庆医科大学生殖生物学研究室 400016) 摘 要:目的 建立早期自然流产蜕膜组织和早期正常蜕膜组织双向电泳圈谱，并观察两者之间蛋白表达的差异。方法 采 用双向电泳技术分离蜕膜组织蛋白，利用专用软件分析所得差异明显蛋白并使用免疫纽化和 Western blotting 验证双向电泳结 果。结果 成功建立了平期自然流产蜕膜组织和早期正常蜕膜组织双向电泳图谱，并鉴定出 9 个差异显著的蛋白。结论 9 个 差异显著的蛋白的功能主要涉及绒毛外滋养细胞的侵袭、自然流产蜕族组织血管的重建以及蜕膜细胞的凋亡等方面，为进一步研 究自然流产发病机制和治疗手段奠定了基础。 关键词:自然流产p 蜕膜z 蛋白质纽学 doi :lO. 3969/j. issn. 1671-8348. 2010.2 1. 006 申图分类号:R714. 21 ;TQ937 文献标识码:A 文章编号: 1671-8348(2010)21-2861-03 PreJiminary screening and identirication of spontaneous abortion decidual tiss田e special protein. CHEN Qian. TAN Bin .WANG Ying-xiong .et a l. (Laboratory 0 f Reproductive Biology •Chongqing Medical University. Chongqing 400016. China) Abstract:Objective To investigate the differential proteomic expressions between the early spontan回us abortion decidual tis- sue and its normal control. Methods Samples of decidual protein were separated by two-dimensional gel electrohporesis(2-DE). The screened differentially protein expressed protein spots were identified by matrix assisted laser desorption/ionization time-of- flight mass spectrometry (MALDI-TOF- M凹.and key of which was verificated by Western blotting and immunohistochemical stai- ning. Results The two dimensional polyacrylarnide gel electrohporesis profiles of decidual tissue protein were successfully estab- lished by 2-DE. According to MALDI-TOF-MS.9 proteins were significant. Conclusion These differential expression proteins mainly refer to invasion of the blastocyst. blood vessel remodeling and cell apoptosis. This can be an experimental basis for further research of the pathoge