金黄色葡萄球菌侵入人脐静脉内皮细胞初期细胞骨架的动态变化.pdfVIP

金黄色葡萄球菌侵入人脐静脉内 皮细胞初期细胞骨架的动态变化 【摘要】 固的 探讨不同毒力的金黄色葡萄球菌(标准菌株 ATCC25923，产肠毒素株 FRS6B) 感染人脐静脉内皮细胞 (HUVEC)初期，细胞骨架动态变化情况。方法 常规方法制备菌液，分别感染 HUVEC， 1 、 2、 4h 后流式细胞仪分析细胞凋亡，应用激 光共黯焦荧光显微镜观察细胞骨架的动态变化。结果 两种不同毒力的金黄色葡萄球菌与 HUVEC 共商 1h 厨即有凋亡细胞发生，共 鞭焦搜微镜观察可见两种细胞作用 1h 后细胞骨架微丝、微瞥均发生了改变，作用 4h 后细胞内聚集大量细菌，细胞骨架微堂堂、微管部 分开始解离。结论 两种金黄色葡辑球菌感染 HUVEC 初期，产肠毒素菌株 FRS6日诱导细胞凋亡明腿高于ATCC25923，细胞骨架的 动~变化存在差异。 【关键词】金黄色葡萄球菌肉皮细胞细胞凋亡细胞骨架微丝微管 Cell apoptosis and cytoskeleton changes of human umbilícal vein endothelial cells induced by Staphylococcal aureus in vitro MIAO Zhengyou , XU Y加g， LI Ping , et al. Center of Prenatal Diagnosis , Jiaxing Institute of Maternity and Child Health , Jíaxing 314001 , China I Abstract) Objective To investigate cell apptosis and the changes of cytoskeleton of human umbilical vein endothelial cells (HUVECs) induced by Staphylococcal aureus strains in vitro. Methods The cultured human umbilical vein HUVECs were infected by Staphylococcal aureus solutions containing standard strain ATCC2593 or enterotoxic strain FRS6日， which were prepared by conventional methods. The apoptotic cells were analyzed by flow cytometry and the changes of cytoskeleton microtubules and microfilaments were observed by confocal fluorescence microscopy 1, 2 and 4 h after tre创ment. Results The apoptotic HUVECs were detected by flow cyωmetry 1 h after treatment of two Staphylococcal aureus strains. The percentages of apoptotic cells were 7.90% and 6.09% after 2 and 4h treatment of ATCC25923 strain , respectively; those were 9.03% and 12.28% for FRS6B treatment. Compared with control group, the percentages of apoptotic cells were significantly higher in treatment groups. Confocal microscopy demonstrated that the cytoskeleton of infected endothelial cells started changes 1 h a行er treatment; and large amount 01 bacteria accumulated , skeleton microtubules and microfilaments were disaggregated 4h after treatment. Conclu