降解水稻秸秆的好氧性丝状真菌菌株的分离筛选与初步鉴定.pdfVIP

安徽农业科学，Joumal of Anhui Agri. Sci. 2013 ,41 (23) :9766 -9768 责任编辑李菲菲责任校对卢瑶 降解水稻秸轩的好氧性丝状真茵茵株的分离筛选与初步鉴定 夏菠1 ，庞立2 ，廖兴华l ，夏延斌1 * (1湖南农业大学食品科技学院，湖南长沙 410128 :2. 湖南农业大学园艺同林学院，湖南长沙 410128 ) 摘要 [目的]对特定生态环境(腐烂的稻草)中的微生物进行分离筛选，并就这些微生物菌株对水稻秸杆的降解能力进行研究和分析。 [方法]利用以微品纤维素粉等只含纤维素成分的原料作唯一碳源的培养基对从稻草秸杆中分离筛选到的7 株可降解稻草秸杆的丝状 真茵茵株进行分离和筛选，通过形态学观察和生理生化特性2 个层次对其进行鉴定。同时，对这些菌株降解水稻秸轩的能力进行了研 究。[结采]试验发现，筛选出的 7 株菌株中有2 株属于多轮青霉主且，2 株属于烟色曲霉组，1 株小抱根霉主且，1 株梨卵形抱纽以及1 株总 状枝毛霉组。其中 1 株烟色曲霉组和 l 株卵形抱纽降解秸杆纤维素能力较强，另有 l 袜烟色曲霉组和 l 株卵形抱纽降解秸秤半纤维素 能力较强。[结论]研究可为微生物菌种降解水稻秸杆的能力研究提供参考。 关键词 水稻秸杆;丝状真菌;分离;鉴定 中图分类号 5216.2 文献标识码 A 文章编号 0517 -6611 (2013 )23 一 09766 -03 Isolation and Identification of Aerobic Filamenωus Fungi that Degrading Rice S时k XIA 80 et aI ( F.ωd Science and Technology College , Hunan Agricultural University , Changsha , Hunan 410128) e Abstract [ Objective] To isolate microo耶nisms in specific ecological environment (rotten straw) , and study the degrading abilities of 由thes酣 mlCroo旧rg惧伊ams degrading rice stalks in certain natural ecological environment. [Result J From straw stalks , 7 strains were isolated. Morphological observation and biochemical identification showed that 2 isolates belong to penicilli um , 2 belong to Aspergillus fumigatus , 1 belongs to Rhizopus micros- porus , 1 Gongronella butleri and 1 Mucor racemosus. Furthermore , the ability of these isolates to degrade rice stalks was studied. Among which , the abilities of degrading straw cellulose of 1 Aspergillus fumigatus and 1 Gongronella butleri are strongger , the abilities of degrading straw hemi- cellulose of another Aspergillus fumigatus and 1 Gongronella butleri are strongger. [Conclusion J The study can provide reference for microbial strains degrading rice