胃舒散对消炎痛所致大鼠小肠损伤的保护作用.docVIP

胃舒散对消炎痛所致大鼠小肠损伤的保护作用 【摘要】 目的： 研究 胃舒散对消炎痛所致大鼠小肠损伤的保护作用及机制。 方法 ： 皮下注射消炎痛(10mg/kg)制作大鼠小肠损伤模型。用光镜、扫描电镜和透射电镜观察不同剂量［30、15、075g/(kg·d)］胃舒散对小肠损伤的 影响 ,同时检测小肠黏膜髓过氧化物酶(MPO)、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量以及血清NO含量。结果：消炎痛引起明显的小肠黏膜损伤，伴有NO、MPO、MDA含量增加及SOD活性下降(P005,P001)。胃舒散剂量依赖性地减少小肠黏膜损伤指数, 提高了胃组织SOD活性(P005)、 降低了MPO、 MDA含量(P005)， 但对血清NO水平无明显影响(P005)。结论：氧自由基及NO参与了消炎痛所致小肠黏膜的损伤，胃舒散对此病变有明显保护作用，其机制与抗氧化作用有关。 【关键词】 小肠损伤 消炎痛 胃舒散 一氧化氮 超氧化物歧化酶 丙二醛 ［Abstract］Objective:To study the protective effect of WEishusan on small intestinal lesions induced by indomethacin in rats. Methods: Wistar rats were injected indomethacin(suspension 10 mg/kg)subcutaneously, and the small intestine was examined for lesions 24 hours later. The different doses［30、15、075 g/(kg·d)］of Weishusan were administered i.g. twice, intestinal mucosal changes were observed by quantitative histology，scanning electron microscopy and transmisson electron microscopy. Superoxide dismutase(SOD)、myeloperoxidase(MPO)activities and malondialdehyde(MDA)levels in intestinal tissues and nitric oxide(NO)levels in serum were determined. Results: Severe lesions in the jejunum and ileum were caused by indomethacinand the NO、MPO and MDA contents were markedly increased(P005). Weishusan dose_dependently prevented the intestinal lesions(P005), enhanced SOD activities(P005)and decreased MPO and MDA levels(P005) but not affected NO levels(P005). Conclusion: Oxygen free radicals and NO may play important roles in intestinal lesions induced by indomethacin. Weishusan can protect the small intestine by antioxidation mechanism. ［Key Words］intestinal lesion; indomethacin; Weishusan; nitric oxide; superoxide dismutase; malondialdehyde 近年，随着老年人口的增多，非甾体类消炎药(NSAID)在防治冠心病、风湿性骨关节炎等疾病中 应用 愈来愈广泛，其导致的胃肠疾病亦逐年增多。胃舒散是我院自行研制的中西药复方散剂，多年临床应用显示对胃炎、消化性溃疡及胃肠功能紊乱等有良好的疗效［1，2］，但是否对药物引起的小肠损伤有 治疗 作用尚不清楚。本项目通过检测有关生化指标并借助光镜、电镜、生化技术观察胃舒散的保护作用，旨在探讨消炎痛致小肠损伤的机理并为临床寻找新的治疗方案。 1材料与方法 11 动物与药物Wistar大鼠［汕头大学医学院实验动物中心提供。体质量(220±35)g，雌雄各半］40只，分笼饲养，自由饮水(过滤除菌的自来水)。胃舒散由我院药剂科生