辣椒PPI蛋白与AGAMOUS-like蛋白间相互作用的鉴定.pdfVIP

辣椒删蛋白与46■加骆like蛋白间 相互作用的鉴定 王晓平 郭金菊 李亚男 王鹏 沈火林+ (中国农业大学园艺学院， 设施蔬菜生长发育调控北京市重点实验室， 北京 100193) 的发育。这有助于进一步揭示辣椒雄蕊发育的分子机理。 关键词辣椒；PPI；PAGl；PAG2；酵母双杂交；双分子荧光互补；蛋白互作 PPI ProteinsinHot IdentificationofInteractionbetweenandAGAMOUS-IikePepper WANG GUO LIYananWAN SHENHuolin+ XiaopingJinju Peng for Protected Growthand of KeyLaboratoryRegulatingVegetableCrop (Beijing Horticulture，China 100193,China) AgriculturalUniversity,Beijing AbstractInthis andbimolecularfluorescence were studyyeasttwo．hybrid usedtotesttheinteractionofClassB AGAMOUS．1ike and contro^ protein(PPr、and protein(PAGlPAG2)which PPI with尉GJ floral inhot resultsshowedthat could interact organdevelopmentpepper．The proteinspecifically andPAG2 whichmeantthatthe尸尸：，andthesetwoAGAMOUS．1ikeinthehot floral respectively proteins pepper couldform toCO—controlthe ofthe stamens．Theis organdevelopmentproteincomplex developmentpepper study the stamen ofcertain forfurther themolecularmechanismof significanceunderstanding pepper development． wordshot two interaction Key pepper；PPI：PAGl；PAG2；yeasthybrid；BiFC；protein 调控花器官的形成中发挥重要作用。Coen【1们、 Alvarez—Buylla…、Theissen【21、Becker【31、 Kaufmann【41等报道MADS—box基因编码蛋白是 一类广泛存在于细菌、真菌、植物和动物中的庞大 说中，所有相关的基因(除了AP2外)几乎都属 转录因子家族，Parenicov{i【51在研