补体ckamcd.pptVIP

* II Cytokine (CK,细胞因子） 1. Concept: --low-molecular-weight proteins (8-80kDa) ---- are polypeptides produced by the cells of innate and adaptive immunity in response to different stimuli. These proteins can mediate many functions of these cells. ---由免疫细胞及非免疫细胞经刺激合成、分泌的一类具有多种生物活性的小分子蛋白质。 * A. 生物学特征 低分子量 (MW 15 ~30KD) 单体多见, 二聚体、三聚体少 快速反应与高效性 (CK binding to CKR, pmol/L) ? 无特异性和MHC 限制性 2.共同特征 * C1s C4a C2 C4b C4 C2a C4b2b C3a C3b C3 C4b2b3b C4b2 C3 convertase C5 convertase Mg2+ Mg2+ * III Membrane attacking stage---- 膜攻击阶段 C4b2b3b 膜攻击复合物(Membrane Attack Complex， MAC)形成 MAC溶解细胞 * C3b C2 b C4b C5 b C5a C5 转化酶裂解 C5。 Microbiology and immunology online, University of south carolina school of medicine /ghaffar/complement.htm * C8 C 9 C 9 C 9 C 9 C 9 C 9 C 9 C 9 C 9 C5 b C6 C7 C5b 依次与 C6, C7, C8,C9 结合，形成膜攻击复合物。 Microbiology and immunology online, University of south carolina school of medicine /ghaffar/complement.htm From: Kuby immunology * IV Biological effects: 参与适应性免疫应答 在抗感染的中晚期或再次免疫应答的发挥效应 * C1 C4a C2 C4b C4 C2a C4b2b C3a C3b C3 C4b2b3b C5a C5b C5 C5b6789 C9 C8 C7 C6 Immune complex C1 Classical Pathway C3 convertase C5 convertase * 由免疫复合物（IC）激活 激活顺序:C1qrs-C4-C2-C3-C5-C6-C7-C8-C9 产生3 个转化酶 产生3个过敏毒素 ：C3a,C4a,C5a 主要特点 * MBL (mannan-binding lectin）pathway （甘露聚糖结合凝集素途径） 由甘露聚糖结合凝集素激活 MBL C4 C4a + C4b + 病原体 MASP2 C4b2b 甘露糖残基 C2 C2a+ C2b MASP: MBL-associated serine protease(MASP1, MASP2) C4b2b: C3 convertase C3 convertase * MBL MBL C1q MBL 为急性期蛋白，与C1q 结构相似。 C1q From: Kuby immunology /courses/immunology/students/spring2006/finley/c3.html * Biological effects: 参与固有免疫应答 在抗感染免疫早期发挥效应 * C3 ? C3b + B factor ?C3bBb ? C3bBb3b C3a Ba 正反馈放大 activate P factor