体液免疫应答网络课案.ppt

Y Y Y Y Y Y B B B B B B Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y T Independent Antigens (TI-1 e.g. LPS) Six different B cells will require 6 different antigens to activate them At high dose TI-1 antigens (like LPS) will POLYCLONALLY ACTIVATE all of the B cells irrespective of their specificity. TI-1 antigens are called MITOGENS LPS complexes with CD14, LPSBP TLR4 TI-2 antigens Only activate mature B1 cells. * TI-1抗原激活成熟和非成熟B细胞 - 高浓度TI-1Ag多克隆激活B细胞 - 低浓度TI-1Ag特异性激活B细胞 * TI-2抗原直接激活成熟的B1细胞 - 其重复决定基与多个BCR结合, BCR交联,产生IgM B细胞对TI抗原的免疫应答 无须Th辅助，无免疫记忆，发生时间早。 小 结 B细胞活化: - 抗原识别信号：BCR单识别, 抗原-C3d复合物交联BCR和共受体， Iga 和Igb传递信号； - 共刺激信号: CD40/B细胞与CD40L/Th细胞结合； - 细胞因子：Th细胞产生。 Th 细胞仅帮助向其提呈特异性抗原的B细胞，提供CD40L和细胞因子。 在生发中心B细胞经历体细胞高频突变和阳性选择，导致抗体亲和力成熟 、抗体类型转换、抗原受体编辑、产生浆细胞和记忆B细胞。 非胸腺依赖性免疫应答: 无需T细胞辅助，无MHC限制性，无免疫记忆，无应答改善。 体液免疫效应: 中和作用，调理作用，激活补体，ADCC效应和黏膜局部免疫应答。 思 考 题 简述B细胞对TD-Ag的初次免疫应答过程。 简述参与初次体液免疫应答的细胞因子。 简述参与T-B细胞相互作用的黏附分子及其功能。 * * 主要针对细胞外病原体 * TD-Ag和TI-Ag * There are two events: the first is recognition of antigen by BCR including single recognition, ligation of coreceptor and signal transduction. The second is antigen process and presentation. * As we know BCR can directly recognize the B cell epitope on native antigen. Because B cell epitopes are conformational determinants and lineal determinants on antigen surface. So BCR recognizes native antigen without antigen processing and MHC restriction. * CD 19 is CR2, its ligand is C3d. The cytoplasma domain of CD19 contains the phosphorylation site for PTK which is associated with signal transduction. If the antigen invade into our body, then firstly trigger the innate immunity, including activation of complement system. Which complement activation pathways are involved in innate immunity? The cross link of corecetor to BCR can increase the sensitivity of BCR to antigen stimulation. Antigens invade into our body and stimulate complement system by activation of alternative pathway and MB