VP-DSC清洗方法.pdfVIP

GE Healthcare Life Sciences Instruction 28-9897-40 AA MicroCal™ VP-Capillary DSC New cleaning protocols for MicroCal VP-DSC system and MicroCal VP-Capillary DSC system New cleaning protocols are described for effective cleaning of DSC B. Rigorous cleaning cells containing high concentrations of aggregated proteins. These In extreme cases when the protein has formed gelatin-like or glue-like cleaning protocols are considerably safer than a concentrated nitric protein aggregates that cannot be removed by the filling syringe. acid solution that is currently used in many laboratories. 1 Add the cleaning solution in small aliquots and heat to 50°C for a The new cleaning solution is made of 5% sodium hydroxide (NaOH) few minutes until the aggregate starts to dissolve. and a sodium hypochlorite (NaOCl) solution. 2 Repeat until all the aggregated protein in the cell is completely Preparation of cleaning solution: dissolved, as judged from the color of the removed solution. 1 Weigh five grams of sodium hydroxide (pellets, ACS reagent 3 Cool the cell to room temperature and rinse well with distilled or grade) and dissolve with 25 ml of de-ionized or distilled water in de-ionized water. a 100 ml volumetric flask. 4 After cleaning, perform a buffer vs. buffer or water vs. water run 2 Add 50 ml of sodium hypochlorite solution (reagent grade, to make sure that the cell is properly cleaned. containing ~ 10-15 % chlorine) to th