细胞计数及细胞活率(国外英文资料).docVIP

细胞计数及细胞活率(国外英文资料) The cell survival rate is calculated Cell living rate is the ratio of living cells, and can be used to count living cells in blue and white. Add 5mL medium to thoroughly mix the cell suspension. Direct the sample to the blue solution. For example, take 20ul cell suspension and add 80ul to the blue solution, and the dilution is five times thinner. Vortex suspension oscillations, mixed dilute cell samples. Preparation of a blood cell counter: first clean the small room and cover glass with alcohol, then dry it with skim. Carefully cover two small rooms with cover glass. A sample of 10ul diluted with a small amount of a liquid is filled with two small Chambers, not overfull or inadequate. Count the total number of cells in the four large squares (1x1x0.1mm). Dead cells are dyed blue dead cells (because of the destruction of cellular integrity), and living cells are transparent (undyed). Cell density calculation: The average number of living cells in each grid is x diluent x104 = living cell count/mL. The percentage of living cells is calculated as follows: Number of living cells (number of living cells + dead cells) x100 % = living cell percentage. The cell counting board USES the method Experimental principle: when the cell suspension under study cells were distributed evenly, by determining the number of a certain volume of the suspension cells, to convert the number of cells per milliliter cells in suspension cells. ? Specific operation:? 1.? Wipe the counters and covers and cover the plate with the cover. ? 2.? The cell suspension suck out a little, add on the edge of the cover plates, between the suspension cover plates and counting board, let stand for 3 min, pay attention to the cover plates under dont have a bubble, also cannot let beside the suspension into the slot. ? 3.? Calculate the total number of cells in the four cells of the cell, and the line cells are only on the left and above. Then the formula is calculated:? Cell count/mL = 4 ce