细胞质膜透性检测实验报告(国外英文资料).docVIP

细胞质膜透性检测实验报告(国外英文资料) The test of nine-plant cytoplasmic membrane permeability One, the purpose of the experiment To understand the damage of high temperature to plant cell membrane; Learn to use conductivity meter Second, the experimental principle The plant cell membrane is a separation barrier, the composition of the cytoplasm and extracellular cell and material exchange of main channel and the bad border cells feel bad about stress of the most sensitive parts. The selective permeability of cell membranes is one of the most important functions. Adversity damage will cause the film choose permeability changes and loss, make the material inside the cells (especially electrolytes) extravasation in great quantities, cause tissue soak liquid conductivity value changes, even suggest that plasma membrane damage degree and the resistance of material measurement size. 3, laboratory equipment and reagents Laboratory instruments and equipment Dds-11a digital inductance meter, temperature box, beaker, measuring tube and tweezers 2, the experimental reagent Distilled water Experimental materials Little begone, orange Four, the experiment step 1, use the punch to remove 20 pieces of small ttang vegetables, 20 slices of orange peel, rinse water several times with distilled water, to remove the material from the wound, reduce the effect of the infiltration of the wound. And then with water paper blot of small siu-tong food and orange skin with water, each material can be divided into two groups, each group of 10, respectively on two small beaker containing 20 ml of distilled water, put a cup of 45 degrees temperature box, the other a under room temperature conditions of 20 to 30 degrees. After 2, 30 minutes, carefully remove the experimental material, cool to room temperature, and measure the conductivity of the solution in each cup with a conductivity meter. Put the material in the beaker, boil it in boiling water for 10 minutes, remove the seedling, cool to room temperature an