当归六黄汤分煎样品与合煎样品中阿魏酸、盐酸小檗碱、黄芩苷含量的比较.docVIP

当归六黄汤分煎样品与合煎样品中阿魏酸、盐酸小檗碱、黄芩苷含量的比较 作者：郑亚玉，梁光义，钟正灵，张丽艳，靳凤云，曹佩雪，杨玉琴，陈添福 【摘要】 目的对当归六黄汤分煎样品与合煎样品中阿魏酸、盐酸小檗碱、黄芩苷的含量进行比较，从而为其临床应用提供依据。方法均采用反相液相色谱法，DIKMA ODSC18柱(5 μm 4.6 mm×250 mm)，以0.085％磷酸－乙腈为流动相梯度洗脱，流速为1.0 ml·min-1，柱温：35，检测波长为326nm测定阿魏酸。以乙腈－0.5%的三乙胺（磷酸调pH=3.00)（2773）为流动相，流速为1.0 ml·min-1，柱温：30，检测波长为343 nm测定盐酸小檗碱。以甲醇水－磷酸(5545∶0.2)为流动相，检测波长为280 nm，流速为1.0ml·min-1，柱温30测定黄芩苷。结果3个指标性成分在分煎样品中平均含量均大于合煎样品中平均含量。结论从中药复方汤剂中指标性成分的含量考虑，当归六黄汤配方颗粒应用于临床具有一定的可行性。 【关键词】 当归六黄汤； 阿魏酸； 盐酸小檗碱； 黄芩苷 China　Abstract：ObjectiveTo compare the contents of ferulic acid、berberine 、baicalin in single medicine decoction and mixed medicine decoction of Danguiliuhuang decoction， and to provide a scientific basis of Danguiliuhuangdecoction for clinic. MethodsThe samples were determined by HPLC. DIKMA ODSC18 column (5 μm, 4.6 mm×250 mm)was used， the mobile phase consisted of 0.085% phosphoricacetonitrile with flow rate 1.0ml·min-1 ， The detection wavelength was set at 326 nm and the column temparature was 35 and Ferulic Acid was detected. The mobile phase consisted of acetonitrile and water（27:73)，which was adjusted to pH3.0 by 0.5％ triethylamine solution and phosphofic acid with flow rate 1.0 ml·min-1 ， the detection wavelength was seted at 343nm ，the column temparature was 30℃and berberine was detected . The mobile phase consisted of methanolwaterphosphoric （55：45：0.2）with flow rate 1.0 ml·min-1. The detection wavelength was set at 280 nm，the column temprature was 30 and baicalin was detected.ResultsThe contents of ferulic acid 、berberine 、baicalin in single medicine decoction was higher than in mixed medicine decoction.ConclusionIt can be used in clinic as a reference. 　　Key words： Dangguiliuhuang decoction；Ferulic acid；Berberine；Baicalin 当归六黄汤出自李东垣《兰室秘藏》，由当归6 g，生地黄6 g，熟地黄6 g，黄连6 g，黄芩6 g，黄柏6 g，黄芪12 g 7味药组成，药材均为粗末。每服五钱（15g），水两盏（300 ml），煎至一盏（150 ml），口服［1］，主治阴虚火旺证。配方颗粒汤剂与传统汤剂的化学成分、药效是否有明显的差异，配方颗粒是否应广泛应用于临床，还存在争论。本文以阿魏酸、盐酸小檗碱、黄芩苷的含量为指标，采用HPLC法比较该方中药配方颗粒（分煎）与传统汤剂（合煎）制得的汤剂中指标性成分的含量是否有差异，从有效成分含量对比方面，为中药配