普罗布考对冠状动脉球囊损伤后管壁原癌基因c-myc表达的影响.docVIP

普罗布考对冠状动脉球囊损伤后管壁原癌基因c-myc表达的影响 [摘要]目的：通过建立猪冠状动脉球囊损伤模型，观察普罗布考对血管内膜增生及管壁原癌基因c-myc表达的 影响 。 方法 ：选用雌性NewYorkshire良种猪19头，随机分为两组，对照组11头，普罗布考组8头，均行冠状动脉球囊扩张术，术后喂饲6周，重复冠状动脉造影后处死动物。普罗布考组于术前4周给予普罗布考1g,bid,至实验结束。常规病理制片，观察形态学变化，采用RT-PCR的方法检测c-mycmRNA的表达强度。结果:对照组损伤动脉内膜可见大量平滑肌细胞增生，呈偏心性增厚，普罗布考组内膜增厚程度明显减低。对照组c-mycmRNA相对表达强度（2.73±0.51）明显高于正常动脉（0.82±0.09，P0.05），普罗布考组c-mycmRNA相对表达强度（1.44±0.25）较对照组减弱（P0.05），但仍高于正常动脉（P0.05）。结论:普罗布考具有抑制冠状动脉球囊损伤后c-myc表达和内膜增生的作用。 [关键词]普罗布考；经皮经腔冠状动脉成形术；c-myc基因 Effectsofprobucolontheexpressionofc-mycmRNAandneointimalhyperplasiaaftercoronaryangioplastyinaswinemodel YUExin1,PANQi-xing1,LIJi-fu1,ZHANGWEi1,TIANFeng-zheng2,LIUChun-sheng1,MADao-xin1,LIGui-shuang1,CHENYu-guo1,YUAi-qin3,MAYu-lin3,YANGKai3 (1DepartmentofCardiology,theQiLuHospitalofShandongUniversity,Jinan250012,China;2 The QiLupharmaceuticalfactory,Jinan250000,China;3TheZichuanhospital,Zibo255000,China) [Abstract]Objective:Toobservetheeffectofprobucolontheexpressionofc-mycmRNAandneointimalhyperplasiaaftercoronaryangioplastyinaswinemodel.Methods:19femaleNewYorkshirepigswererandomlyassignedtorecEIve2g.d-1ofprobucolorcontroltherapyfor4weeksbeforepercutaneoustransluminalcoronaryangioplasty(PTCA),thenPTCAwasunderwentinallthepigs,Probucolwascontinueduntilfollow-upangiography6weeksafterPTCA.Thentheanimalswerekilledandtakenhistopathologicexamination.RT-PCRmethodwasusedtodetecttheexpressionofc-mycmRNAintheinjuredarteries.Results:Theinjuredarteryofthecontrolgrouphadeccentrichyperplasticneointimaandtheexpressionofthec-mycmRNA(2.730.51)washigherthanthenormalartery(0.820.09,P0.05),Theinjuredarteryoftheprobucolgrouphadlesshyperplasticneointimaandlowerexpressionofthec-mycmRNA(1.440.25)thanthecontrolgroup(P0.05),buthigherthanthenormalartery(P0.05).Conclusion:Probucolcandecreasetheexpressionofc-mycmRNAandtheneointimalhyperplasiaafterPTCA. [Keywords]Probucol；Percutaneoustransluminalcoronaryangioplasty；C-mycgenes, 　　近年的 研究 表明抗氧化剂普罗布考（probucol,丙丁酚）具有抑制经皮经腔冠状动脉成形术(percutaneoustransluminalcoronaryangioplasty,PTCA)后再狭