基于ISSR方法的芦笋育种亲本间亲缘关系分析.pdf

热带亚热带植物学报 2017, 25(2): 149 ~ 156 Journal of Tropical and Subtropical Botany 基于ISSR 方法的芦笋育种亲本间亲缘关系分析 林宗铿, 林艺华, 蔡坤秀, 郑涛 (福建省热带作物科学研究所, 福建 漳州 363000) 摘要：为了解芦笋(Asparagus officinalis L.)育种亲本间的遗传关系, 采用ISSR 方法对19 份芦笋育种亲本进行亲缘关系和遗 传多样性分析。结果表明, 18 条引物共扩增出187 条谱带, 多态性比率达93.6% 。UPGMA 聚类分析表明, 亲本的相似系数为 0.56~ 0.80, 在相似系数 0.62 处可将亲本分成 3 大类群, 第Ⅰ类群为美国芦笋品种„绿龙‟; 第Ⅱ类群由荷兰品种„金林‟人工培 育的部分亲本和美国品种„格兰德‟组成; 第Ⅲ类群为其余亲本组成。同一品种不同个体间也可能存在较大的遗传距离, 芦笋 经倍性育种培育出的个体表现出丰富的遗传多样性。这为芦笋杂交育种中的亲本筛选提供了理论依据。 关键词：芦笋; 育种; 亲缘关系; ISSR doi: 10.11926/jtsb.3644 Genetic Relationships among 19 Breeding Parents of Asparagus by ISSR LIN Zong-keng, LIN Yi-hua, CAI Kun-xiu, ZHENG Tao (Fujian Institute of Tropical Crops, Zhangzhou 363000, Fujian, China) Abstract: In order to understand the genetic relationship among of Asparagus officinalis L. germplasms, the genetic diversity of 19 asparagus parents were analyzed by ISSR. The results showed that 187 bands were amplified by 18 primers with polymorphism rate for 93.6%. UPGMA indicated the Nei‟s coefficient ranged from 0.56 to 0.80. These cultivars could be clustered into three groups according to average coefficient of 0.62. Asparagus officinalis „Green Dragon ‟ cultivar from US formed Group Ⅰwhich had far relation with others. Group II consisted of some artificial breeding parents of „Gijnlim‟ from Holland and „Grande‟ from US. The other tested parents made up the Group III. The individuals derived from the same variety had large genetic distance. There were abundant genetic diversity among individuals of asparagus from ploidy breeding. Therefore, these would provide the theoretical basis and technical guidance for parent selection in hybrid breeding of asparagus. Key words: Asparagus; Breeding; Genetic relat