补阳还五汤类方成分药代动力学比较探讨.docVIP

补阳还五汤类方成分的药代动力学比较研究 　　摘要：目的 比较补阳还五汤类方（补阳还五汤和脑健片）中阿魏酸、洋川芎内酯A、藁本内酯在大鼠体内的药代动力学特征。方法 将补阳还五汤类方灌胃颈静脉插管大鼠，采用HPLC-DAD法同时检测3种成分在不同时间点的血药浓度，经DAS2.0计算得单成分的药代动力学参数，再采用总量统计矩（相似度）法整合成整体药代动力学参数并进行比较。结果 3种成分在体内的总量统计参数相差甚远，而3种成分总和的统计矩参数却比较接近，补阳还五汤和脑健片在大鼠体内AUC分别为240.6和133.0，MRT分别为3.192 min和3.259 min，VRT分别为21.59 min2和19.75 min2；补阳还五汤和脑健片中3种成分总量的相似度达到0.977 8。结论 补阳还五汤和脑健片在体内代谢过程具有相似性，中药复方发挥功效依赖于多成分的整体贡献 关键词：药代动力学；补阳还五汤；脑健片；阿魏酸；洋川芎内酯A；藁本内酯 DOI：10.3969/j.issn.1005-5304.2016.07.021 中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2016）07-0082-05 Comparative Study on Pharmacokinetics of Components of Buyang Huanwu Associated Prescriptions LIU Fang1， YANG Yan-tao1， HE Fu-yuan1， LIU Lin2， WU Shuang1， WANG Yu-hong2， 3， CAI Guang-xian3 （1. Pharmacy School， Hunan University of Chinese Medicine， Changsha 410208， China； 2. The First Hospital of Hunan University of Chinese Medicine， Changsha， 410007 China； 3. National Key Laboratory Co-built by Departments of Herbal Powder and New Drugs of Hunan Province， Traditional Chinese Medicine Powder Technology and Equipment of National and Local Joint Engineering Laboratory， Changsha 410208， China） Abstract： Objective To study the pharmacokinetic features of ferulaic acid， senkyunolide A and ligustilide in Buyang Huanwu associated prescriptions （Buyang Huanwu Decoction and Naojian Tablets）. Methods HPLC-DAD was applied for simultaneous determination plasma concentration of three ingredients with jugular venous cannula rats after intragastric administration of Buyang Huanwu associated prescriptions. The pharmacolinetic parameters of each ingredient was calculated by DAS2.0， and then the total quantum statistical moment （TQSM） standard similarity was used to measure the overall pharmacokinetics behaviors. Results There were great differences in the three ingredients after the administration of two prescriptions， while the total quantum statistical parameters were very closely. The TQSM pharmacokinetic parameters of the three components in Buyang Huanwu Decoc