人鼻腔嗅粘膜嗅鞘细胞的原代培养和免疫组化观察.PDF

2008 年 08 月 生物骨科材料与 临床研 究 第 卷 第 期 ORTHOPAEDIC BIOMECHANICS MATERIALS AND CLINICAL S . . 5 4 7 文章编号：swgk2008-06-0076 论著 ·实验研究 人鼻腔嗅粘膜嗅鞘细胞的原代培养和免疫组化观察* 常 瑞 梅 强 阴小龙 程 延 [ ] ( ) 摘要 目的 建立人鼻腔嗅粘膜嗅鞘细胞OECs 的体外培养方法。 方法 利用鼻窦镜选取上鼻甲嗅区粘膜，原代培 养嗅鞘细胞，进行形态学观察。 结果 成功培养出人鼻腔嗅粘膜OECs，为扁平的双极、多极形态，对P75、GFAP 呈阳性 反应，纯度在50%左右。 结论 人鼻腔嗅粘膜培养OECs 的方法实用可行，但须在取材部位和纯化方面进一步探讨。 [ ] 关键词 嗅粘膜；嗅鞘细胞；原代培养 [ ] + [ ] 中图分类号 Q813.1 文献标识码A primary cultures and immunocytochemical observe of Olfactory ensheathing cells from human olfactory mucosa ChangRui ，MeiQiang ，Yin Xiaolong.YinXiaolong.Department of Orthopedics, XiDian GroupHospital.Xian,7 10077 [Abstract] Obj ective To establish the primary culture methods of Olfactory ensheathing cells from human olfactory mucosa . Methods OECs were acquired by Endoscopy from olfactory mucosa in superior turbinate, cultured and stu- died the morphological characteristics of OECs. Results OECs can be cultured from human olfactory mucosa, appear- ed long bipolar or unbipolar fusiform cell and identified with P75 and GFAP ．The purity is 50%. Conclusion OECs can be acquired from human olfactory mucosa. The position of olfactory mucosa and purification required to investigation. [ ] ( ) Key words Olfactory mucosa；Olfactory ensheathing cells OECs ；Primary culture OECs 在修复脊髓损伤中的潜在作用已被近年来的研