乙酰肝素酶：侵袭与转移的一个关键酶(综述).pdfVIP

Heparanase: A Key Enzyme in Invasion and Metastasis of Gastric Carcinoma Weihua Tang, M.D., Yasushi Nakamura, M.D., Ph.D., Masahiko Tsujimoto, M.D., Ph.D., Misako Sato, M.T., Xiaojuan Wang, M.D., Kazushi Kurozumi, M.D., Ph.D., Masaaki Nakahara, M.D., Kazuyasu Nakao, M.D., Misa Nakamura, Ph.D., Ichiro Mori, M.D., Ph.D., Kennichi Kakudo, M.D., Ph.D. Department of Pathology (WT, YN, MS, XW, MiN, IM, KeK), Wakayama Medical University, Wakayama, Japan; and Departments of Pathology (MT) and Surgery (KaK, MaN, KN), Osaka Police Hospital, Osaka, Japan One of the characteristics of malignant cells is me- Previous reports have shown that the biochemical tastasis, whereby cancer cells invade parenchymal activity of heparanase is significantly correlated tissue and penetrate vascular channels to form sat- with the invasion and metastasis of malignant cells ellite tumors in distant organs. In this process, the in vitro. Recently, it was found that the human basement membrane (BM) and extracellular matrix heparanase gene was cloned and highly expressed (ECM) play a barrier to prevent tumor cells from in malignant cell lines and human solid malignant invasion and metastasis (1, 2). The previous reports tumors. In the present study, we investigated the have shown that heparanase, produced by malig- heparanase mRNA expression by using in situ hy- nant tumor cells, can mediate the degradation of bridization in 116 paraffin-embedded tissues of pri- heparan sulfate proteoglycans (HSPGs) in ECM and mary gastric carcinomas. To explore its clinicopath- BM (3–5) and is one of the key enzymes involved in ologic significance, it was detected in the various the invasion and metastasis of malignant tumors steps of