甘油预处理对冻干红细胞恢复率和抗氧化酶活性的影响.pdf

Effects of Glycerol Pretreatment on Recovery and Antioxidant 1 Enzyme Activities of Lyophilized Red Blood Cells 1 1 1 1* 2 Xinli Zhou ，Hui He ，Baolin Liu ，TseChao Hua ，Ying Chen 1 Institute of Biothermal Sciences ，University of Shanghai for Science and Technology ，Shanghai （200093 ） 2Institute of Blood test ，Shanghai Antu Hospital，Shanghai （200093 ） ∗E-mail ：tchua@ Abstract Successful storage of red blood cells (RBCs) by freeze-drying technique has important implications in blood transfusion and clinical medicine. We presented a method of preservation RBCs by pretreating them with glycerol solution and then freeze-drying. The effects of glycerol pretreatment on recovery and antioxidant enzyme activities of lyophilized RBCs were investigated. RBCs pretreated in 40% glycerol resulted in 55.3±4.26% numerical recovery and 53.5±3.85% hemoglobin recovery, which were significantly different from control group (P0.01). Superoxide dismutase(SOD), catalase (CAT) and glutathione peroxidase (GSH-PX) activities of 40% glycerol pretreated, freeze-dried RBCs almost remained fully active after freeze-drying. After 180 days storage, they decreased by 25.6% ，21.5% and 18.4%, respectively, which are significantly lower than those of no glycerol pretreated, freeze-dried RBCs (P0.1). These data demonstrated that glycerol pretreatment had