玉米秸秆还田土壤中纤维素分解菌的分离筛选和鉴定_英文_.pdfVIP

Agricultural Science ＆ Technology ，2010 ，11 (4):134 － 137 ，147 Copyright  2010 ，Information Institute of HAAS． All rights reserved． Resources and Environment Iso lat io n ，Sc reening and Ident if icat io n of Cellulose Deco mpos ing St rains in St raw － amended So il GUO Yan1，2 * ，ZHA NG J in-liang 1 ，WA NG Hui-ping 1 ，QI Hui1 ，LIA NG Feng 1，2 1． Department of Life Science ，Shangqiu Normal University ，Shangqiu 476000 ;2 ． Engineering Technology Research Center of Biomass Deg- radation and Gas-forming ，University of Henan Province ，Shangqiu 476000 Abst ract ［Objective］The aim was to isolate cellulose decomposing strains with high activity from straw-amended soil． ［Method］Screening of CMC solid culture and shaking culture rescreening were carried out to obtain cellulose decomposing strains with high activity from the corn straw amended soil，which were analyzed by 16rDNA sequence analysis． ［Result］A bacterium and a fungus with higher endonucleases activity were obtained through preliminary screening and secondary screening ，a fungus with higher filter paper enzyme activity ，and a bacterium (NO． 5 strain)with higher of both filter paper enzyme activity and CMC enzyme activity were obtained． The result of 16S rDNA sequence analysis showed that the similarity was 100% between NO． 5 strain and Bacillus subtilis． ［Conclusion］NO． 5 strain was identified as Bacillus subtilis． Key wo rds Straw-amended soil ;Cellulose decomposing strains ;Screening ;Cellulase Straw is a kind of by-product of agricultural production engineered strains ［12］，and the exploring and optimizing of cel- that has a largest number in the world ，which is a kind of crop lulose-producing conditions a