马尾松成熟合子胚的体细胞胚胎发生和植株再生.pdfVIP

1995, 37(4) : 289294 A cta Botanica Sinica * 黄健秋　卫志明　许智宏 ( , 200032) ( P inus mass oniana Lamb.) , 2, 4-D 10 mg/ L, 4 / ( KT BA mg L DCR ) 2, 4- 1. 0 / , 0.4 / D mg L KT BA mg L DCR 9000 mg/ L DCR ABA , 35. 1% , ; ; ; SOMATIC EMBRYOGENESIS AND PLANTLET REGENERA- TION FROM CALLUS OF MATURE ZYGOTIC EMBRYOS OF MASSON PINE H ang Jian-qi , Wei Zhi-ming and X Zhi-hong ( N ational L aboratory of P lant M olec ular Ge netic s, S hanghai Institute of P lant P hy siology , A cad emia S inic a, Shanghai 200032) Abstract Embryogenic c lt res w ere initiated from mat re zygotic embryos of masson pine ( . ) 2, 4- 10 / , P inus massoniana Lamb on DCR medi m s pplemented w ith D mg L KT 4 / . and BA each at mg L Pale and transl cent calli w ith early stage proembryos w ere maintained and m ltiplicated on DCR medi m s pplemented with 2, 4-D 1. 0 mg/ L KT and BA each at 0. 4 mg/ L. Rob st late-stage proembryos were obtained w hen the calli 9000 / - . w ere c lt red on DCR medi m containing mg L myo inositol Abscisic acid and ac- tivated charcoal promoted the formation of cotyledonary embryos at the highest freq en- : 1994-07-12: 1994-09-12 * 290 37 cy of 35. 1%. M at re somatic embryos co ld germinate and develop f rther into plantlets - . w hen they were isolated and c lt red on a hormone free DCR medi m Key w rdsP inus massoniana; Mat re zygotic emb