生物酶振荡器研究谷胱甘肽的抗氧化效应.PDF

2002 年第 60 卷 化 学 学 报 Vol. 60 , 2002 第 4 期 , 715～719 ACTA CHIMICA SINICA No. 4 , 715 ～719 生物酶振荡器研究谷胱甘肽的抗氧化效应 甘南琴　　蔡汝秀 　　林智信　　杨树涛 ( 武汉大学化学系 　武汉 430072) 摘要 　组装了一种生物酶振荡器(PeroxidaseOxidase Biochemical Oscillator) 的实验装置、建立了相应的实验方法 ,在此 ( ) 基础上得到单倍周期、四倍周期、混沌等丰富的振荡图谱信息 ,并采用它作为“探针”,检测了谷胱甘肽 GSH 的抗氧 化效应 ,它可作为定量分析的基础 ,线性范围为 9. 38 ×10- 7 ～7. 50 ×10- 5 mol ·L - 1 , 探讨了某些可能的反应机理. 关键词 　生物酶振荡器 ,PO 振荡反应 ,谷胱甘肽 Study on the Antioxidation Effect of Glutathione Based on the PeroxidaseOxidase Biochemical Oscillator GANG, NanQin 　　CAI , RuXiu 　　LIN , ZhiXin 　　YANG, ShuTao ( Department of Chemistry , Wuhan University , Wuhan 430072) Abstract 　Complex dynamics have become increasingly important in biochemical and physiological contexts in recent years. A good example for studying the nonlinear and nonequilibrium dynamics is the peroxidase oxidase (PO) reaction. The PO reaction involves the oxidation of reduced nicotinamide adenine dinucleotide (NADH) by molecular oxygen and overall reaction catalyzed by horseradish peroxidase ( HRP) . The nonlinear behavior of this reaction is usually realized in a reactor containing the HRP , methylene blue (MB) , and 2 ,4 dichlorophenol (DCP) . NADH and O are