右美托咪定及舒芬太尼联合鞘内注射对CCI模型大鼠的镇痛效应.pdfVIP

ANALGESIC EFFECTS OF DEXMEDETOMIDINE COMBINED WITH SUFENTANIL INTRATHECALLY INJECTION ON CCI MODEL IN RATS Abstract Objective: To study the analgesic effect of dexmedetomidine combined with sufentanil intrathecal injection on CCI model of rats. Methods: Intrathecal catheters were successfully inplanted in 50 male SD rats. All the rats randomly were divided into five groups as follows: Sham group (Sham); model control group (CCI group); dexmedetomidine group (Dex group); sufentanil group (Suf group); combined treatment group (DS group). Rats in Dex group, Suf group and DS group were intrathecally injected respectively with dexmedetomidine 2 mg/(kgd) , sufentanil 1 mg/d and dexmedetomidine 1 mg/(kgd) + sufentanil 0.5 mg/d daily at 1～14 d after CCI. The total capacity of each group of drugs was diluted into 20 ml, rats in CCI group were intrathecally injected with an equal volume of 0.9% saline daily. The paw mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) were measured 30 min after administration before operation and 1, 3, 7, and 14 days after CCI ．Five in each group were sacrificed at 7 and 14 days after operation and the expressions of Fos protein in the dorsal root ganglion (DRG) were examined with the immunohistochemical methods. Results: The MWT and TWL at each time points were significantly decreased and c-Fos protein expressions in DRG 7 days post-operation were significantly higher than group CCI, Dex, Suf and DS compared with group Sham (P0.05). MWT and TWL were significantly increased and c-Fos protein expressions in DRG were significantly lower in group Dex, Suf and DS compared with group CCI (P0.05). MWT and TWL significantly increased and c-Fos protein expressions de