大鼠骨髓间充质干细胞对继发性骨质疏松性骨折的实验研究.docVIP

骨髓间充质干细胞对继发性骨质疏松大鼠骨缺损愈合的影响 高翔1，陈广谋2，黄成硕2，刘志军2，曾荣2，楚佳奇1，李鹏1△ （1广东医科大学附属医院干细胞研发与细胞治疗中心， 广东湛江 524001； 2广东医科大学附属医院微创骨科研究室，广东探讨（Bone mesenchymal stem cells，BMSCs）BMSCs细胞，并使用流式细胞仪检测及使用成骨成脂特异性染色鉴定培养的BMSC；选取60只雌性SD大鼠，通过肌注地塞米松（DEX，1mg/（kg·d））8周,建立大鼠骨质疏松模型。所有大鼠经手术于胫骨平台下钻孔造骨缺损模型，将大鼠随机分成三组：对照组骨折不处理组、DEX骨折+生理盐水（NS）对照组、BMSCs组Micro-CT检测各组大鼠的骨缺损愈合情况。结果 经流式细胞表型鉴定及成骨成脂特异性染色实验均证实分离得到的细胞是BMSCs。Micro-CT结果表明，与DEX骨折+NS组相比，BMSC细胞治疗能促进DEX诱导的骨质疏松性骨折的愈合。结论 BMSCs对继发性骨质疏松性大鼠的骨缺损具有显著的促进愈合作用，这为临床治疗继发性骨质疏松个体的骨损伤提供了一种新的研究思路。 关键词 骨质疏松；骨髓间充质干细胞；骨缺损 The Effect of bone marrow-derived mesenchymal stem cells on the rats of Secondary osteoporosis bone defect healing Gao Xiang1 ,Chen Guangmou2,Huang Chengshuo2,Liu Zhijun2, Zeng Rong2, Chu Jiaqi1,Li Peng1△ (1.Stem Cell Research and Cellular Therapy Center, Affiliated Hospital of Guangdong Medical College, Zhanjiang, 524001 2.Department of Orthopedic Surgery, The Affiliated Hospital of Guangdong Medical College, Zhanjiang, 524001) Abstract: Objective To assess the therapeutic effect of bone marrow-derived mesenchymal stem cells (BMSCs) on osteoporotic bone defect healing. Methods BMSCs were isolated by the whole bone marrow adherence method, then assessing general biological characteristics of BMSCs byflow cytometry and staining of osteoblasts and adipocytes. Sixty female SD rats were intramuscularly injected of dexamethasone (DEX, 1mg/（kg·d）) 8 weeks to establishing rat model of osteoporosis. All the rats were defects in the hole tibial plateau fracture model outside subsequently divided into three groups, i.e., control group fracture is not treated, DEX fracture + saline(NS, control group),DEX fracture + BMSCs treatment group. BMSCs were injected into the rat bone defect site for treatment.( saline as control group). At 4 or 8 weeks, Micro-CT detected BMD of the rats in each group and the observation of bone defect healing in rats. Results Flow cytometry analysis, alizarin red and oil red O staining revealed that isolated BMSCs were positive. Micro-C