肿瘤靶向性药物载体叶酸-壳聚糖微球的制备与特性研究.pdfVIP

南方医科大学学报pSouthMed ·2183- 2008；28(12) Univ) 肿瘤靶向性药物载体叶酸．壳聚糖微球的制备及特性研究 龚金兰。汪森明，胡喜钢，曹漫明，张积仁(南方医科大学珠江医院肿瘤中心，广东广州510282) 摘要：目的探索叶酸偶联壳聚糖(FA．CTS)纳米粒的合成方法及对肿瘤细胞的靶向性。方法利用叶酸活性酯与壳聚 糖分子上的氨基反应形成稳定的酰胺键，制备FA．CTS。再利用壳聚糖在偏酸性溶液中带正电荷，在高速磁力搅拌作用 光标记法和MTT法评价其肿瘤细胞靶向性。结果所制备的FA．CTS／盯X纳米粒，形态均质规则。无粘连，平均粒径 282．8 FA—CTS纳米粒可通过叶酸受体途径特异靶向于叶酸受体丰富的肿瘤细胞。 关键词：叶酸偶联壳聚糖纳米粒；紫杉醇；纳米粒；药物载体 中图分类号：R73．3 文献标识码：A 文章编号：1673-4254(2008)12．2183．04 andcharacterizationoffolic chitosan asa Synthesis acid—conjugatednanoparticles carrier tumor．targeteddrug GONG Ji-ren． Jin．1an，WANGSen—ruing，HUXi—gang，CAOMan—ming，ZHANG Medical 1 University，Guangzhou，50282，China OncologyCenter,ZfiujiangHosiptal，Sourthem To andcharacterize Abstract：Objectivesynthesize paclitaxel(PTX)一loadedfolate-conjugated its invitro．MethodsCTS／PTXandFA—CTS／PTX were andevaluate nanoparticles cytotoxicity nanoparticlespreparedusing reductiveamidationandioinc ofchitosanwith sizewasdetermined gelation tripolyphosphateanions(TPP)．Theparticle by laser andthe observedtransmissionelectron andthePTXcontentinthe scattering morphologyusing microscopy, wasdeterminedultraviolet at227am．Theinvitro ofthe nanoparticles using spectrophotometer cytotoxicitynanoparticles HeLacellsWasevaluatedMTT WasusedtoobservetheHeLacellsincubatedwith assay．Fluorescence against by microscopy in aci